MAPREC与MNVT评价Ⅲ型Sabin株脊髓灰质炎病毒神经毒力的比较  

Evaluation of the neurovirulence of type Ⅲ Sabin strain poliovirus through MAPREC and MNVT

作　　者：李娜 丁玲 马萌 孙千一 王红燕 李拓 LI Na;DING Ling;MA Meng;SUN Qian-yi;WANG Hong-yan;LI Tuo(Department of Quality Control,Beijing Bio-Institute Biological Products Co.,Ltd.,Beijing 100176,China;Department of Vaccine Facility 4,Beijing Bio-Institute Biological Products Co.,Ltd.,Beijing 100176,China)

机构地区：[1]北京生物制品研究所有限责任公司质量检定室,100176 [2]北京生物制品研究所有限责任公司疫苗4室,100176

出　　处：《中国医药生物技术》2019年第6期494-499,共6页Chinese Medicinal Biotechnology

基　　金：“重大新药创制”国家科技重大专项(2018ZX09737008)

摘　　要：目的用PCR扩增及限制性酶切分析突变技术(MAPREC)和猴体神经毒力试验(MNVT)评价同一批Ⅲ型Sabin株脊灰病毒神经毒力的结果差异。方法采用MAPREC技术,检测Ⅲ型Sabin株脊灰病毒神经毒力关键位点472位点突变率;采用MNVT病理切片分析,评价Ⅲ型Sabin株脊灰病毒神经毒力。结果 MAPREC检测待测样品核酸472位点突变率均值为0.324%,低于高突变参考品的0.621%。MNVT切片结果分析表明,xtest–xref=0.169,小于C1。对同一批样品,MAPREC与MNVT分析结果一致。结论在Ⅲ型Sabin株脊灰病毒神经毒力检测方面,操作简单,试验周期短的MAPREC技术可作为金标准MNVT的有益补充。Objective To study the difference between the neurovirulence of the same type Ⅲ Sabin strain poliovirus examined by MAPREC and MNVT. Methods The MARPEC assay was used to detect the point mutation rate of the 472 site, which are the key sites of the virulence of the type Ⅲ Sabin strain poliovirus. The paraffin sections of the MNVT was analyzed to evaluate the neurovirulence of the type Ⅲ Sabin strain poliovirus. Results The average mutation rate of 472 site in MAPREC assay was 0.324%, which was lower than 0.621% of HMVR. The paraffin sections results of MNVT showed that xtest xref = 0.169, and the result was less than C1. The results of MAPREC of the same batch of samples were consistent with the results of MNVT. Conclusion In the detection of virulence of poliovirus type Ⅲ Sabin strain, the simple operation and short test period of MAPREC can be used as a beneficial supplement to the gold standard MNVT.

关 键 词：神经毒力 Ⅲ型Sabin株脊髓灰质炎病毒 分子水平 动物水平 

分 类 号：S85[农业科学—兽医学]