分子印迹固相萃取-流动注射化学发光法测定蔬菜中甲基对硫磷  

Determination of methyl parathion in vegetables by molecularly imprinting solid phase extraction-flow injected chemiluminescence method

作　　者：陈文梅 侯建国 Chen Wenmei;Hou Jianguo(Zhejiang Geological Prospecting Institute,China Chemical Geology and Mine Bureau,Hangzhou 310000,China)

机构地区：[1]中化地质矿山总局浙江地质勘查院

出　　处：《分析仪器》2019年第6期71-74,共4页Analytical Instrumentation

摘　　要：本研究中以丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为引发剂,合成了甲基对硫磷分子印迹聚合物,制备而成分子印迹固相萃取小柱。以此作为甲基对硫磷分子识别载体,利用鲁米诺-过氧化氢-甲基对硫磷化学发光体系,建立了测定甲基对硫磷的高选择性的流动注射化学发光分析方法。在8.0×10^-8~6.0×10^-5 mol L-1浓度范围内对甲基对硫磷具有良好的线性关系,线性相关系数为0.9969,检测限为3.4×10^-8 mol L^-1。对5.0×10^-7 mol L^-1的甲基对硫磷溶液进行11次平行测定,相对标准偏差为3.32%。此方法用于蔬菜样品中甲基对硫磷的测定,结果满意。Methyl parathion molecularly imprinted polymer microspheres were synthesized and made into a molecular imprinted solid phase column extractor.Combining with chemiluminescent technology,an imprinting-flow-injection chemiluminescence method for the determination of methyl parathion was established.The linear range is 8.0×10^-8-6.0 × 10^-5 mol L^-1 and the detection limit is 3.4 × 10^-8 mol L^-1.The relative standard deviation of methyl parathion is 3.32%(n= 11).This method has been successfully applied to the determination of methyl parathion residued in vegetable samples.

关 键 词：化学发光 甲基对硫磷 分子印迹聚合物 

分 类 号：R28[医药卫生—中药学]