TSA联合HSV-Ⅰ型对C6细胞增殖、凋亡的影响  

Effects of TSA combined with HSV-1 on proliferation,apoptosis in C6 cells

作　　者：许强华[1] 刘甜[2] 陈新军[3] 陈治军[1] 刘平非[1] 谢腾[1] 陈晓巍[1] XU Qiang-hua;LIU Tian;CHEN Xin-jun(Department of Neurosurgery,The First People's Hospital of Jingmen,Jingmen 448000,China;不详)

机构地区：[1]湖北省荆门市第一人民医院神经外科,荆门448000 [2]湖北省荆门市第一人民医院眼科,荆门448000 [3]武汉大学中南医院神经外科

出　　处：《临床神经外科杂志》2019年第6期472-475,共4页Journal of Clinical Neurosurgery

基　　金：荆门市引导性科技项目(YDKY2015007)

摘　　要：目的研究曲古抑菌素A(TSA)联合单纯疱疹病毒Ⅰ型(HSV-1)对肿瘤细胞增殖、凋亡的影响。方法以0.5×10^-3μmol/L TSA和10MOI HSV-1及0.5×10^-3μmol/L TSA与10MOI HSV-1联合作用于C6鼠胶质瘤细胞;分别于48 h、72 h后,用CKK-8法检测各组细胞的增殖活性,流式细胞仪检测各组细胞凋亡率。结果48 h后,TSA+HSV-1组C6细胞较单一TSA组、单一HSV-1组C6细胞的增殖活性率明显降低(P<0.05,P<0.01);72 h后,TSA+HSV-1联合组C6细胞较单一TSA组和单一HSV-1组C6细胞的增殖活性率明显降低(均P<0.01)。48 h、72 h后,TSA+HSV-1组C6细胞较单一TSA组和单一HSV-1组C6细胞的凋亡率明显升高(均P<0.01)。结论TSA联合HSV-1对体外培养的C6细胞具有协同或叠加杀伤作用。Objective C6 cells are cultured in vitro as models to observe kojic ancient bacteriostatic A(TSA)combine typeⅠherpes simplex virus(HSV-1)effects on tumor cell proliferation and apoptosis.Methods C6 cells in vitro were treated separately with 0.5×10^-3μmol/L TSA,10MOI HSV-1 and 0.5×10^-3μmol/L TSA combined with 10MOI HSV-1 for 48 h and 72 h.The CCK-8 assay was used to observe the proliferation of C6 cells.The rate of apoptosis was detected by Flow Cytometry.Results The proliferation activity rate of TSA+hsv-1 group was lower than that of TSA group and HSV-1 group 48 h later(P<0.05,P<0.01).After 72 h,the proliferation activity rate of C6 cells in the TSA+HSV-1 combined group was significantly lower than that in the TSA group and the HSV-1 group(all P<0.01).At 48 h and 72 h,the apoptosis rate of C6 cells in TSA+HSV-1 group was significantly higher than that in TSA group and HSV group(all P<0.01).Conclusion TSA combined with HSV-1 has synergistic or superimposed killing effect on C6 cells cultured in vitro.

关 键 词：曲古抑菌素A 单纯疱疹病毒Ⅰ型 胶质瘤 增殖 凋亡 

分 类 号：Q2-33[生物学—细胞生物学]