与脑心肌炎病毒衣壳蛋白相互作用宿主蛋白的筛选  

Screening of host proteins interacting with encephalomyocarditis virus capsid proteins

作　　者：谢晶莹[1] 邓盈盈[2] 韩玉梅[2] 张海霞[1] 李向茸[1] 李琼毅[2] 冯若飞[1,3] XIE Jing-ying;DENG Ying-ying;HAN Yu-mei;ZHANG Hai-xia;LI Xiang-rong;LI Qiong-yi;FENG Ruo-fei(Key Bio-engineering and Technology Laboratory of the Northwest Minzu University,Lanzhou 730030;Life Science and Engineering College of Northwest Minzu University,Lanzhou 730030;Gansu Engineering Research Center for Animal Cell,Lanzhou 730030,China)

机构地区：[1]西北民族大学生物工程与技术国家民委重点实验室,兰州730030 [2]西北民族大学生命科学与工程学院,兰州730030 [3]甘肃省动物细胞工程技术研究中心,兰州730030

出　　处：《生物学杂志》2019年第6期20-24,共5页Journal of Biology

基　　金：国家自然科学基金项目(31460665);教育部“长江学者和创新团队发展计划”项目(IRT_17R88)

摘　　要：应用Dual hunter酵母双杂交系统,以脑心肌炎病毒(Encephalomyocarditis virus,EMCV)衣壳蛋白VP1、VP2和VP3为诱饵,通过对BHK-21细胞cDNA文库的筛选,共得到24个互作蛋白。经过生物信息学分析,发现其功能包括蛋白结合、催化活性、转运活性和结构分子活性;参与生物调节、细胞形成、细胞代谢、发育过程、细胞定位和免疫反应等多个生理过程,并参考文献资料分析这些蛋白在病毒复制中可能起到的作用,为研究EMCV感染及其致病机理提供参考。In order to investigate the biological function of encephalomyocarditis virus(EMCV)capsid protein VP1,VP2 and VP3 in virus infection.Capsid proteins were constructed as baits to screen the cDNA library of BHK-21 cells by Dual hunter yeast two-hybrid system.24 proteins have been identified to interact with EMCV proteins.Through bioinformatics analysis,it was found that its functions include binding,catalytic activity,transporter activity and structural molecule activity.They were involved in a series of biological processes,such as biological regulation,cellular component organization or biogenesis,metabolic process,developmental process,localization and response to stimulus.The possible roles of these proteins in viral replication were analyzed with reference to literatures,which may provide reference for further study of EMCV infection.

关 键 词：脑心肌炎病毒 衣壳蛋白 宿主蛋白 筛选 

分 类 号：Q81[生物学—生物工程] S852.65[农业科学—基础兽医学]