天津地区日本沼虾群体遗传多样性的微卫星分析  

Genetic Diversity Analysis of Wild Macrobrachium nipponense Populations from Tianjin Based on Microsatellite

作　　者：王永辰[1] 姜巨峰[1] 刘肖莲[1] 宋立民 吴会民[1] 李楠[1] 马林[1] 高哲颖 WANG Yongchen;JIANG Jufeng;LIU Xiaolian;SONG Limin;WU Huimin;LI Nan;MA Lin;GAO Zheying(Tianjin Fishery Research Institute,Tianjin 300221,China;Hangu Fishery Technical Extension Station,Tianjin 300480,China)

机构地区：[1]天津市水产研究所,天津300221 [2]天津市汉沽区水产技术推广站,天津300480

出　　处：《天津农业科学》2019年第12期37-43,共7页Tianjin Agricultural Sciences

基　　金：天津市科技计划项目(18ZXZYNC00150);天津市水产研究所创新团队项目(scyjs201903)

摘　　要：为了评估天津地区日本沼虾(Macrobrachium nipponense)野生种质资源遗传背景,采用9对微卫星分子标记(Mn33、Mn37、Mni04、Mni01、Mni06、Mni76、Mni13、Mni40、Mni58)对日本沼虾4个野生群体(YDXH、DLJH、XQLH和YQSK)进行了遗传多样性研究。结果表明,9对微卫星引物在日本沼虾4个群体中的等位基因数(Na)为3~12,有效等位基因数(Ne)为1.9769~10.8165,观测杂合度(Ho)为0.2857~0.9750,期望杂合度(He)为0.4942~0.8967,多态信息含量(PIC)为0.4749~0.8880,说明日本沼虾群体的遗传多样性水平较高;分子方差分析(AMOVA)显示,群体中仅有2.04%(P<0.01)的遗传变异来源于群体间,97.96%(P<0.01)的变异来源于群体内,表明遗传差变异主要存在于个体间;群体间遗传分化指数(Fst)为0.0104~0.0377,说明群体间的遗传分化程度并不明显;基于群体间Nei’s遗传距离采用UPGMA法对4个群体进行聚类树构建,永定新河(YDXH)与独流减河(DLJH)首先聚为一支,其次与西七里海(XQLH)聚为一支,最后与于桥水库(YQSK)聚为一支。In order to investigate the genetic background of wild Macrobrachium nipponense resources in Tianjin,nine microsatellite markers(Mn33,Mn37,Mni04,Mni01,Mni06,Mni76,Mni13,Mni40,Mni58)were used to analyze the genetic diversity of four populations of M.nipponense(YDXH,DLJH,XQLH and YQSK).The results demonstrated that number of alleles(Na)was 3~12,number of effective allele(Ne)was 1.9769~10.8165,observed heterozygosity(Ho)was 0.2857~0.9750,expected heterozygosity(He)was0.4942~0.8967 and polymorphic information content(PIC)was 0.4749~0.8880,indicating that the four populations had a high genetic diversity.AMOVA analysis revealed that 2.04%of the total genetic variation was among populations and 97.96%was among individuals.Genetic differentiation index(Fst)(0.0104~0.0377)suggested that there was low genetic differentiation among these populations.The UPGMA phylogenetic tree revealed that YDXH and DLJH clustered into one group,then to XQLH,finally to YQSK.

关 键 词：天津地区 日本沼虾 微卫星 遗传多样性 

分 类 号：S966.12[农业科学—水产养殖] Q347[农业科学—水产科学]