鹿源枯草芽孢杆菌对小鼠生长性能、细菌易位、血清生化指标和血液学指标的影响  

Effects of the Treatment of Bacillus subtilis Isolated from Sika Deer on the Growth Performance,Bacterial Translocation,Serum Biochemical Indices and Hematological Indices in Mice

作　　者：王卓[1] 刘晓颖[1] 李光玉[1] 郭肖兰 王晓旭[1] 张贵贤 王殿永 刘晗璐[1] 邱宏坤 WANG Zhuo;LIU Xiao-ying;LI Guang-yu;GUO Xiao-lan;WANG Xiao-xu;ZHANG Gui-xian;WANG Dian-yong;LIU Han-lu;QIU Hong-kun(State Key Laboratory of Special Economic Animal Molecular Biology,Institute of Special Wild Economic Animals and Plants,Chinese Academy of Agricultural Sciences,Changchun 130112,China;Agricultural and Rural Bureau of Changli County,Qinhuangdao 066600,China;Animal Disease Prevention and Control Center in ChangliCounty,Qinhuangdao 066600,China;Hengdaohezi Northeast Tiger Forst Park,Hailin City,Heilongjiang Province,Hailin 157114,China)

机构地区：[1]中国农业科学院特产研究所吉林省特种经济动物分子生物学省部共建国家重点实验室,长春130112 [2]河北省昌黎县农业农村局,河北秦皇岛066600 [3]河北省昌黎县动物疫病预防控制中心,河北秦皇岛066600 [4]黑龙江省海林市横道河子东北虎林园,黑龙江海林157114

出　　处：《特产研究》2019年第4期47-52,共6页Special Wild Economic Animal and Plant Research

基　　金：国家重点研发计划资助项目(2018YFC1706600);中国农业科学院科技创新工程专项(CAAS-ASTIP-2018-ISAPS);中国农业科学院基本科研业务费项目(201720);吉林省科技发展计划项目(20160307022NY)

摘　　要：对鹿瘤胃内分离鉴定的枯草芽孢杆菌D-12菌株的安全性进行评价,以确定其是否能作为安全、可食用的益生菌,并用于后续产品研发。通过小鼠连续口服枯草芽孢杆菌D-12株28 d喂养毒性试验,观察小鼠行为、活动、饮食、粪便等体征,统计小鼠体重和采食量,计算脏器指数,并对其血液生化指标、细菌易位、血液学指标进行检测。结果显示,小鼠无异常表现;各组小鼠体重、采食量、内脏指数和血清生化指标均无显著差异,血液学指标正常,无细菌易位现象。枯草芽孢杆菌D-12对小鼠安全、无毒副作用。To assess the safety of Bacillus subtilis D12 strain isolated from the rumen of sika deerwhether it is edible probiotics for the growth ofanimals,a28-day feeding toxicity test was set up in mice.The behavior,activity,diet,feces and other general appearances,body weight,counted the body weight and feed intake,calculated the organ index,and detected blood biochemical indices,bacterial translocation and haematological indices were observed closely during whole experiment.The results demonstrated that mice had no abnormalities during the test period.There was no significant difference in body weight,feed intake,visceral index and serum biochemical indices among different groups.The hematological indices were normal,there was no bacterial translocation.Thus B.subtilis D-12 issafe and non-toxic to mice.

关 键 词：鹿源枯草芽孢杆菌 小鼠 生长性能 细菌易位 血液生化指标 血液学指标 

分 类 号：S852.616[农业科学—基础兽医学]