两种检测水貂阿留申病毒抗体方法敏感性的比较研究  

Comparative Studies on the Sensitivity of Two Methods in Detecting the Antibody Against Aleutian Mink Disease Virus

作　　者：王振军[1] 罗国良[1] 易立[1] 郭利[1] 冯二凯[1] 程世鹏[1] 程悦宁[1] WANG Zhen-jun;LUO Guo-liang;YI li;GUO Li;FENG Er-kai;CHENG Shi-peng;CHENG Yue-ning(Institute of Special Wild Economic Animals and Plants,Chinese Academy of Agricultural Sciences,Changchun 130112,China)

机构地区：[1]中国农业科学院特产研究所

出　　处：《特产研究》2019年第4期57-63,共7页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技发展计划项目(20190301086NY)

摘　　要：本研究对胶体金试纸条检测法与对流免疫电泳检测法检测水貂阿留申病毒抗体的敏感性进行对比研究。试验采用实验室制备的检验合格的试纸条,分别对20份不同效价的水貂阿留申病毒阳性质控血清和100份自然感染的田间血清进行检测,分析不同稀释度的阳性血清检出数量和阳性检出率,从而对两种检测方法的敏感性进行评估。结果显示,阳性质控血清在1:64倍以下稀释时,试纸条对阳性血清和弱阳性血清的检出率均为100%,对100份自然感染阳性血清检出率为96%,表明该试纸条的检测具有较高的敏感性。In this study,the sensitivity of colloidal gold strip test and CIEP for detection of antibody against Aleutian mink disease virus was compared.20 samples of AD positive quality control serum with different titers and 100 samples of naturally infected field serum were tested by using the qualified test strips prepared in the laboratory in this experiment,.The sensitivity of the two methods was evaluated by analyzing the number and detection rate of positive serum with different dilution.The results showed that when the positive quality control serum was diluted below 1∶64,the detection rate of positive serum and weak positive serum was 100%,and the detection rate of 100 natural infection positive sera was 96%.The results show that the test strip has high sensitivity.

关 键 词：胶体金试纸条 对流免疫电泳 水貂阿留申病毒抗体 敏感性 

分 类 号：S858.922.659.2[农业科学—临床兽医学]