慢性心力衰竭大鼠脑血流量变化及其一氧化氮合酶作用机制  

Study on Cerebral Blood Flow in Rats with Chronic Heart Failure and Mechanism of NOS

作　　者：陆梓雯 赵明镜[1] 王蕾[1] 杨涛[1] 赵一舟[1] 仇琪[2] 成文堃 李彤[1] 王保福 杨京京 李洋 蒋洋洋 LU Ziwen;ZHAO Mingjing;WANG Lei;YANG Tao;ZHAO Yizhou;QIU Qi;CHENG Wenkun;LI Tong;WANG Baofu;YANG Jingjing;LI Yang;JIANG Yangyang(Key Laboratory of Chinese Internal Medicine Ministry of Education and Beijing,Institute of Cardiovascular Diseases,Qi-Blood Research Laboratory of Integrated Traditional Chinese and Western Medicine,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100700,China;不详)

机构地区：[1]北京中医药大学东直门医院中医内科学教育部和北京市重点实验室、北京中医药大学心血管病研究所、中西医结合气血研究实验室,北京100700 [2]首都医科大学安贞医院

出　　处：《中西医结合心脑血管病杂志》2019年第21期3312-3317,共6页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：北京市自然科学基金重点项目资助(No.7161008)

摘　　要：目的探讨慢性心力衰竭后大鼠脑血流量的变化及其一氧化氮合酶作用机制。方法采用结扎左冠状动脉前降支造成心肌梗死后慢性心力衰竭模型,60 d后采用超声心动检测心功能;通过Morris水迷宫定向航行实验和穿台实验评价认知功能;使用激光散斑多普勒血流仪检测脑血流量;通过免疫印迹法检测大鼠脑皮质内皮型一氧化氮合酶(eNOS)与诱导型一氧化氮合酶(iNOS)的含量。结果与假手术组相比,模型组射血分数(EF)和短轴缩短率(FS)明显降低(P<0.05),心功能下降;与假手术组相比,模型组定向航行实验的潜伏期与总路程差异均无统计学意义(P>0.05),穿台实验中穿台次数明显下降(P<0.01);与假手术组相比,模型组脑血流量差异无统计学意义(P>0.05);模型组大鼠大脑皮质eNOS表达量与假手术组相比有降低趋势,但eNOS/iNOS比值明显下降(P<0.05)。结论慢性心力衰竭大鼠存在明显的认知功能障碍,主要表现为记忆力下降。尽管慢性心力衰竭大鼠与血管舒张和收缩相关的一氧化氮合酶系统已出现异常,但是其脑部血流量并无明显变化。Objective To explore the changes and mechanisms of cerebral blood flow in rats after chronic heart failure.Methods The model of chronic heart failure after myocardial infarction was established by ligating anterior descending branch of the left coronary artery.The echocardiography was used to detect cardiac function after surgery for 60 days.The cognitive function was evaluated by acquisition trial and probe trial of Morris water maze.Cerebral blood flow was measured by laser speckle Doppler flowmeter.The contents of eNOS and iNOS in the cerebral cortex were detected by western blotting.Results Compared with the sham group,EF and FS values in the model group significantly decreased(P<0.05)and cardiac function was decreased.There was no significant difference between the sham group and model group in the average latency and path length of acquisition trial(P>0.05),however,the number of crossing the platform significantly reduced(P<0.01).Cerebral blood flow did not change in heart failure rats compared with that in the sham group(P>0.05).The eNOS expression of the model group was lower than that in the sham group,and eNOS/iNOS ratio decreased significantly(P<0.05).Conclusion The rats with chronic heart failure had obvious cognitive dysfunction,which was mainly manifested as the decline of the memory.Although the NOS system associated with vasodilation and contraction was impaired,however the cerebral blood flow of chronic heart failure rats did not change.

关 键 词：慢性心力衰竭 认知功能障碍 脑血流 内皮型一氧化氮合酶 诱导型一氧化氮合酶 射血分数 短轴缩短率 

分 类 号：R541.6[医药卫生—心血管疾病] R285.5[医药卫生—内科学]