悬浮培养型BHK-21细胞生长特性及成瘤性研究  

Growth Characteristics and Tumorigenic of Suspension Cultured BHK-21 Cells

作　　者：赵彩红 王美皓[1,2] 臧荣鑫 乔自林[1] 马忠仁[1,2] 王家敏[1,2] ZHAO Cai-hong;WANG Mei-hao;ZANG Rong-xin;QIAO Zi-lin;MA Zhong-ren;WANG Jia-min(Gansu Tech Innovation Center of Animal Cell,Biomedical Research Center,Northwest Minzu University,Lanzhou,730030,China;Life Science and Engineering College,Northwest Minzu University,Lanzhou,730030,China)

机构地区：[1]西北民族大学生物医学研究中心甘肃省动物细胞技术创新中心,甘肃兰州730030 [2]西北民族大学生命科学与工程学院,甘肃兰州730030

出　　处：《西北民族大学学报（自然科学版）》2019年第4期53-59,共7页Journal of Northwest Minzu University(Natural Science)

基　　金：西北民族大学中央高校基本科研业务费专项资金项目(31920150029);西北民族大学中央高校基本科研业务费专项资金资助研究生项目(Yxm2018143);甘肃省重点研发计划(18YF1FA121)

摘　　要：目的:了解低血清驯化的悬浮培养型BHK-21细胞的生长特性及致裸鼠成瘤能力.方法:比较研究悬浮型和贴壁型BHK-21细胞的细胞形态、生长动力学和成瘤性.结果:驯化的悬浮培养型和贴壁培养型BHK-21细胞生长状态均良好,最大增殖浓度分别为517×10^4/mL和39.67×10^4/mL,群体倍增时间分别为23.03 h和21.36 h,能显著提高细胞培养密度(P<0.05).背部皮下接种致裸鼠成瘤性实验表明,驯化的悬浮培养型组裸鼠的体重增长率均低于贴壁培养型BHK-21细胞、阳性细胞组(Hela)和阴性细胞组(KMB17)(P>0.05),裸鼠成瘤体积增长率均高于其他三组(P<0.05).结论:驯化的悬浮培养型BHK-21细胞能显著提高细胞密度,其成瘤性方面还需进一步改善.Objective In order to understand the growth characteristics and the tumorigenic ability to nude mice in suspension cultured BHK-21 cells,which was low serum acclimation in our laboratory,Method The cell morphology,growth kinetics and tumorigenicity of domesticated suspension cultured and adherent cultured BHK-21 cells were studied.Result The results showed that the adherent culture and domesticated suspension culture BHK-21 cells grew well,the maximum proliferation concentrations were 39.67×10^4/mL and 517×10^4/mL,respectively,and the population doubling time was 23.03 h and 21.36 h,respectively.After two subcultures of BHK-21 cells and positive cells(Hela cells)and negative cells(KMB17 cells)were subcutaneously inoculated into nude mice,the growth rate of the nude mice in the domesticated suspension culture group was lower than that of the other three groups.At the same time,the growth rate of the tumor volume of the nude mice was higher than that of the other three groups,and the final tumor volume was also significantly different from the final tumor volume of the nude mice in the positive group and the adherent group(P<0.05).Conclusion Therefore,suspension culture of BHK-21 cells can significantly increase cell density,in terms of suspension culture of BHK-21 cells'tumorigenicity,further improvement is needed.

关 键 词：驯化后悬浮培养型BHK-21细胞 贴壁培养型BHK-21细胞 生长特性 成瘤性 

分 类 号：Q813.1[生物学—生物工程]