MUCT工艺反硝化除磷效率与Candidatus Accumulibacter的群落演替  

Efficiency of Denitrifying Phosphorus Removal and Population Dynamics of Candidatus Accumulibacter in MUCT

作　　者：曾薇 李超 孟庆安 马晨阳 ZENG Wei;LI Chao;MENG Qing'an;MA Chenyang(National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,Engineering Research Center of Beijing for Denitrifying Phosphorous Removal and Process Control,Beijing 100124,China)

机构地区：[1]城镇污水深度处理与资源化利用技术国家工程实验室北京市污水脱氮除磷处理与过程控制工程技术研究中心

出　　处：《北京工业大学学报》2019年第12期1243-1251,共9页Journal of Beijing University of Technology

基　　金：国家自然科学基金资助项目(51578016)

摘　　要：以处理实际生活污水的连续流MUCT工艺为基础,探究了全程硝化和短程硝化状态下优势聚磷菌Candidatus Accumulibacter的群落动态与系统反硝化除磷效果之间的相互联系.流式细胞仪定量检测结果表明,系统中聚磷菌占全菌的比例为22.1%,Accumulibacter是处于全程硝化状态下的优势聚磷菌,对系统中氮磷的去除起到重要作用.q PCR定量结果表明,短程硝化状态下的优势种IIC和IIC(ppk1 excluding OTU NS D3)以及IIF的增长率较快,占总Accumulibacter比例分别为11.53%、34.31%和17.97%,其利用亚硝进行反硝化除磷的能力比其他分支强,对系统化中亚硝的去除起到重要作用.IID分支在反应器整个运行阶段一直处于优势地位,是系统内氮磷去除最重要的分支.其在全程硝化状态下占Accumulibacter分支总和的80%,但在短程状态下的竞争能力不如其他的TypeⅡ型分支.In this study,Modified University of Cape Town(MUCT)process was used to treat domestic sewage with lowρ(C)/ρ(N)ratio,and the relationship between population dynamics of dominant Candidatus Accumulibacter and efficiency of denitrifying phosphorus removal under nitration and nitrification was studied.Flow cytometry based quantification showed that Accumulibacter accounting for22.1%of total bacteria were dominant phosphate accumulating organisms(PAOs)in system under nitrification.qPCR based estimation indicated that dominant species IIC,IIC(ppk1 excluding OTU NS D3)and IIF grew fast and accounted for 11.53%,34.31%and 17.97%of Accumulibacter,respectively,while their ability using nitrite to achieve denitrifying phosphorus removal were stronger than that of other clades and were important for nitrite removal in system.As the most important clade in N and P removal in system,IID was always dominant along entire operation,which accounted for 80%of Accumulibacter under nitrification,but not as competitive as other TypeⅡclades under nitration.

关 键 词：实际生活污水 反硝化除磷 聚磷菌 Accumulibacter 实时荧光定量PCR 流式细胞仪 

分 类 号：X703.1[环境科学与工程—环境工程]