组织培养中玻璃化现象的研究进展  

Research progress of vitrification in plant tissue culture

作　　者：赵一鸣 隋宝凤 张欣燕 侯凯琳 刘柏玲[1] ZHAO Yi-Ming(College of Life Science,Qufu Normal University,Shandong Qufu 273165,China)

机构地区：[1]曲阜师范大学生命科学学院

出　　处：《国土与自然资源研究》2019年第6期68-74,共7页Territory & Natural Resources Study

基　　金：国家自然科学基金(31700624);曲阜师范大学博士启动基金(bsqd20130136);曲阜师范大学大学生创新创业训练计划项目(201910446102)

摘　　要：组织培养过程中出现的玻璃化现象是限制其发展和组培苗商业化生产的三大主要影响因素之一。据统计,组织培养过程中出现玻璃化的植物种类目前已达200多种。本文就玻璃化苗的形态解剖特征和生理生化特点,影响玻璃化产生的因素,玻璃化产生的生理生化变化和基因表达差异,以及如何预防和减少玻璃化的产生等方面展开论述,并指出了在玻璃化研究中待解决的一些问题,期望为组织培养的玻璃化研究提供一定的参考价值。Vitrification is one of the three main factors to limit the development of tissue culture technology and the commercialized production of seedlings in plant tissue culture.So far,there have been more than 200 species with vitrification in plant tissue culture.In this review,we introduced the morphological and anatomical features of the hypherdric plantlets,discussed the physiological and biochemical characteristics of the vitrification shoots,and analyzed the physiological and biochemical changes and gene expression differences between the normal and vitrification shoots.Additionally,we reviewed some methods to reduce the incidence of vitrification and recover the hyperdric plantlets to normal ones.We also summarized some questions which have not been researched in present studies of vitrification.It is expected that this review would provide some reference for the research of vitrification in plant tissue culture.

关 键 词：组织培养 玻璃化 生理变化 基因表达 

分 类 号：Q813.12[生物学—生物工程]