基于粪便DNA的贺兰山岩羊亲权鉴定和婚配制研究  

Parentage verification and mating system of Pseudois nayaur in Helan Mountains Based on Faecal DNA

作　　者：祝招玲 刘振生[1,3] 高惠[1] 张致荣 姚绪新 刘鹏[1] 韩兴志 王继飞 滕丽微[1,3] ZHU Zhaoling;LIU Zhensheng;GAO Hui;ZHANG Zhirong;YAO Xuxin;LIU Peng;HAN Xingzhi;WANG Jifei;TENG Liwei(College of Wildlife and Protected Area,Northeast Forestry University,Harbin 150040,China;College of Economics and Management,Jiamusi University,Jiamusi 154007,China;Key Laboratory of Conservation Biology,State Forestry Administration,Harbin 150040,China;Helan Mountains National Nature Reserve of Ningxia,Yinchuan 750021,China)

机构地区：[1]东北林业大学野生动物与自然保护地学院,哈尔滨150040 [2]佳木斯大学经济与管理学院,佳木斯154007 [3]国家林业局野生动物保护学重点开放实验室,哈尔滨150040 [4]宁夏贺兰山国家级自然保护区管理局,银川750021

出　　处：《生态学报》2019年第22期8639-8647,共9页Acta Ecologica Sinica

基　　金：中央高校基本科研业务费资助项目(2572014CA03);国家林业和草原局野生动植物保护与自然保护区管理司项目;国家自然科学基金项目(31372221,31870512)

摘　　要：2017年7—8月和11—12月在贺兰山岩画风景区采集到369份岩羊新鲜粪便样本,用多态性较高的10个微卫星位点进行基因分型,共识别出282只岩羊个体。通过复合扩増和牙釉蛋白基因PCR法共鉴定出152只雄性,130只雌性。微卫星分析得到平均等位基因(Na)为10.1,平均观测杂合度(Ho)为0.7737,平均期望杂合度(He)为0.6512,平均多态信息(PIC)为0.6129。运用Cervus3.0软件进行亲权鉴定,并用Kingroup V2计算个体间的亲缘系数(r),作为亲权鉴定的补充,共鉴定出父 母 子7对,父 子21对,母 子19对。根据亲缘关系对岩羊的婚配制进行推测分析,表明岩羊婚配制应属一雄多雌。We tested for parentage verification and mating systems in Pseudois nayaur in Helan Mountain Rock Painting Scenic area.369 fresh faecal samples were collected from July to August and November to December 2017.All faecal samples were then tested through genotyping,using 10 microsatellite loci that had relatively high polymorphism.A total of 282 individuals(152 males and 130 females)were identified through compound amplification and amelogenin gene(AMELX&AMELY)PCR.A total of 101 alleles were detected in 10 microsatellite loci and the average number of alleles(Na)was 10.1,while the mean observed and expected heterozygosities were 0.7737 and 0.6512.Furthermore,the mean value of polymorphic information contents(PIC)was 0.6129.Parentage verification was conducted on Cervus 3.0.Kingroup V2 and was used to calculate the coefficient of relationship(r)between individuals as a supplement to parental identification.The results showed that there were 7 parentage groups,21 father child groups and 19 mother child groups.Based on the analysis of these relationships,we deduced that Pseudois nayaur have a polygynous mating system.

关 键 词：岩羊 亲权鉴定 婚配制 贺兰山 

分 类 号：Q95[生物学—动物学]