铁皮石斛、流苏石斛及紫皮石斛中总多糖含量测定研究  

Content Determination of Total Polysaccharides in Dendrobium Officinale, Dendrobium Fimbriatum and Dendrobium Devoninum

作　　者：陈丹 吴梅青 张广宁 李金科 李柯[2] CHEN Dan;WU Meiqing;ZHANG Guangning;LI Jinke;LI Ke(Xiangtan Medicine Health Vocational College,Xiangtan 411102,China;Hunan Academy of Traditional Chinese Medicine,Changsha 410013,China)

机构地区：[1]湘潭医卫职业技术学院,湖南湘潭411102 [2]湖南省中医药研究院,湖南长沙410013

出　　处：《中国民族民间医药》2019年第22期50-54,共5页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：湖南省教育厅项目(17C1569)

摘　　要：目的:建立铁皮石斛、流苏石斛及紫皮石斛中总多糖的含量测定方法。方法:采用紫外分光光度法,以无水葡萄糖为对照品,在483nm波长处测定铁皮石斛、流苏石斛及紫皮石斛中总多糖的含量。结果:无水葡萄糖在1.742~12.191μg·mL-1质量浓度范围内具有良好的线性关系,r=0.999 3;平均回收率为99.8%,RSD为3.29%。铁皮石斛中总多糖含量在35.64~39.39g·100g-1之间,流苏石斛总多糖含量在9.25~12.82g·100g-1之间,紫皮石斛总多糖含量在32.57~37.27g·100g-1之间。结论:不同品种含量差异较大,采用紫外分光光度法测定石斛中总多糖含量的方法操作简便、快速,具有较好的专属性和准确性。Objective To establish a method for content determination of total polysaccharides in Dendrobium officinale, Dendrobium fimbriatum and Dendrobium devoninum. Methods The content of total polysaccharides in Dendrobium officinale, Dendrobium fimbriatum and Dendrobium devoninum was determined by ultraviolet spectroscopy with anhydrous glucose as reference substance at 483 nm wavelength. Results Anhydrous glucose showed good linearity in the range of 1.742~12.191 μg·mL-1(r=0.999 3), and the average recovery rate(n=6)of the anhydrous glucose was 99.8%, and RSD was 3.29%. The content ranges of polysaccharides in Dendrobium officinale, Dendrobium fimbriatum and Dendrobium devoninum in different batches were 35.64~39.39 g·100 g-1, 9.25-12.82 g·100 g-1, 32.57-37.27 g·100 g-1. Conclusion There was large difference in content of different varieties, the method of determining the total polysaccharide content in Dendrobium by ultraviolet spectrophotometry is simple and rapid, and has good specificity and accuracy.

关 键 词：铁皮石斛 流苏石斛 紫皮石斛 无水葡萄糖 紫外分光光度法 

分 类 号：R284.1[医药卫生—中药学]