干旱胁迫对马尾松菌根化苗木水分生理和光合特性的影响  

Effect of Water Stress on Physiology and Photosynthesis Properties of Ectomycorrhizal Pinus massoniana Seedlings

作　　者：王艺 张弛[1] 丁贵杰 黎之骞[1] WANG Yi;ZHAN Chi;DING Gui-jie;LI Zhi-qian(College of Forestry,Guizhou University,Guiyang 550025,China;College of Ecological Environment and Engineering,Guizhou Minzu University,Guiyang 550025,China)

机构地区：[1]贵州民族大学生态环境工程学院,贵州贵阳550025 [2]贵州大学林学院,贵州贵阳550025

出　　处：《浙江林业科技》2019年第4期1-8,共8页Journal of Zhejiang Forestry Science and Technology

基　　金：国家青年科学基金项目（31400537）;贵州省科学技术基金项目（黔科合J字﹝2013﹞2143号）;贵州省教育厅青年科技人才成长项目（黔教合KY字﹝2018﹞134）;贵州民族大学自然科学基金;贵州省大学生创新创业项目

摘　　要：以马尾松Pinus massoniana 1年生苗为材料,采用双因素随机区组试验设计,6个接种处理,分别为牛肝菌7 Suillus luteus,牛肝菌1,鸡油菌Cantharellus cibarius,彩色豆马勃Pisolithus tinctorius Strain Pt 715,土生空团菌Cenococcum geophilum Strain Cg SIV和对照(不接种);4个水分处理,土壤相对含水量分别为正常(65±5)%、轻度胁迫(50±5)%、中度胁迫(35±5)%和重度胁迫(25±5)%,比较分析马尾松菌根化及非菌根化苗木,在干旱胁迫下水分生理和光合特性的差异。结果表明,外生菌根真菌通过提高叶片相对含水量,增加束缚水含量,降低苗木水势、自由水和水分饱和亏缺,从而改善苗木水分状况,增强苗木保水能力;外生菌根真菌增加了苗木叶绿素含量,提高了苗木光合速率,各处理间差异显著(P<0.05)。较高的叶绿素含量使菌根化苗木的光合速率增强,气孔导度、胞间CO2浓度和资源利用效率也提高,从而降低蒸腾速率、气孔限制值和光合耗氧量,减少气孔对水分的阻力,提高菌根化苗木的含水量;马尾松菌根化苗木主要是通过提高光合利用效率来适应干旱环境,其抗旱能力由强到弱为:牛肝菌1>土生空团菌>牛肝菌7>鸡油菌>彩色豆马勃>对照。Experiment were conducted by random design of two factors on 1-year Pinus massoniana seedlings inoculated by Suillus luteus SL7&SL1,Cantharellus cibarius,Pisolithus tinctorius Strain Pt 715,Cenococcum geophilum Strain Cg SIV with different soil relative water content(65±5)%,(50±5)%,(35±5)%and(25±5)%.The result showed that ectomycorrhizal fungi could increase relative ans bound water content of leaves,reduce seedling RWC,free water and water saturation deficit to improve seedling moisture to strengthen the drought resistance of seedlings.Ectomycorrhizal seedlings had higher chlorophyll content and photosynthetic rate by promoting the absorption of nutrients with significant difference between different treatments.The photosynthetic rate,stomatal conductance,intercellular CO2 concentration and efficiency of resources use was improved by higher chlorophyll content of ectomycorrhizal seedlings by reducing the transpiration rate,stomatal limitation and photosynthetic oxygen consumption,stomatal resistance of moisture,and increasing water content.Drought resistant of ecotmycorrhizal seedlings was followed by S1>T>S7>G>C>CK.

关 键 词：马尾松 菌根化苗木 水分生理 光合特性 抗旱性 

分 类 号：S791.248[农业科学—林木遗传育种]