齿轮状SBA-15的制备及其对葡萄糖氧化酶的吸附行为研究  

The Synthesis of Gear-like SBA-15 Particles and Its Adsorption Behavior on Glucose Oxidase

作　　者：庄凯[1] 何光春[1] 何全文[1] ZHUANG Kai;HE Guangchun;HE Quanwen(Sichuan Technology and Business College,Dujiangyan 610083,Sichuan,China)

机构地区：[1]四川工商职业技术学院

出　　处：《陶瓷学报》2019年第5期631-636,共6页Journal of Ceramics

基　　金：四川工商职业技术学院博士基金(BS1701)

摘　　要：以非离子表面活性剂P123为模板剂、正硅酸乙酯TEOS为硅源,在酸性条件下,水热合成了齿轮状SBA-15介孔材料,考察了水热合成温度对齿轮状SBA-15介孔材料孔径的影响。当水热合成温度从100℃升高至160℃,齿轮状SBA-15介孔材料的孔径从5.95 nm增大至9.22 nm。以合成的SBA-15介孔材料为载体负载葡萄糖氧化酶,考察了体系pH值,载体材料孔径对吸附量的影响。吸附体系的pH值将影响载体材料和酶的电荷特性,当pH值为4时载体材料与葡萄糖氧化酶酶带异种,载酶量较大;孔径是影响载酶量的另一关键因素,当介孔材料孔径为&77 nm时,载酶量达到最大值128 mg/g。Gear-likeSBA-15 particles were synthesized in aqueous acid conditions using amphiphilictriblock copolymer Pluronic P123 as the template and tetraethoxysilane TEOS as the silica source,and the hydrothermal temperature was controlled to expand the pore-diameter.With the hydrothermal temperature increased from 100℃ to 160℃,the pore-diameter was expanded from 5.95 nm to 9.22 nm.The synthesized SBA-15 particles were applied to immobilizeglucose oxidase,and the effects of the system pH valueand the pore-diameter on immobilization capacity were investigated.When the pH value was 4,the carrier material and the glucose oxidase enzyme were heterogeneous charged,the enzyme immobilization capacity was larger;the pore-diameter wasanother key factor of the enzyme immobilization capacity,and the maximum amount of enzyme load reached 128 mg/g with the pore-diameter of 8.77 nm.

关 键 词：SBA-15 齿轮状 水热温度 孔径 载酶量 

分 类 号：TQ174.75[化学工程—陶瓷工业]