液相色谱—串联质谱法测定番茄和黄瓜中敌菌灵残留量  

Determination of anilazine in tomatoes and cucumbers by LC-MS/MS

作　　者：陈景春 CHEN Jing-chun(Shanghai Bino Testing Service Co.,Ltd.,Shanghai 201114,China)

机构地区：[1]上海必诺检测技术服务有限公司

出　　处：《食品与机械》2019年第11期75-78,共4页Food and Machinery

摘　　要：样品采用固相萃取小柱净化,以C18色谱柱为分离柱,以甲酸水-乙腈为流动相进行梯度洗脱,经LC-MS/MS检测,以多反应监测模式检测目标化合物的特征离子进行定性定量分析。结果表明:该方法线性范围为0.1~10.0μg/mL,线性相关系数>0.999 0,方法的检出限为0.01mg/kg,定量限为0.03mg/kg。在加标水平为0.03,0.10,0.50 mg/kg时,方法的回收率为88.3%~95.2%,RSD在4.5%~8.8%。该方法前处理方式简便、有机溶剂用量少、分析时间短、选择性好且灵敏度高,适用于番茄和黄瓜中敌菌灵的检测。Purified by solid phase extraction column,using C18 column as separation column and formic water-acetonitrile as flow phase for gradient elution,qualitative and quantitative analysis of characteristic ions of target compounds were detected by LC-MS/MS in MRM mode.Results,this method was in the linear range of 0.1~10.0μg/mL,linear correlation coefficient of>0.999 0,the detection limit of the method was 0.01 mg/kg,and the quantitative limit was 0.03 mg/kg.At the spiking levels of 0.03,0.10,and 0.50 mg/kg,the recovery rate of the method was88.3%~95.0%,with RSD was 5.2%~8.8%.The method has the advantages of simple pretreatment,low amount of organic solvent,short analysis time,good selectivity and high sensitivity.The method is suitable for the detection of anilazine in tomatoes and cucumbers.

关 键 词：高效液相色谱-串联质谱法 敌菌灵 番茄 黄瓜 残留量 

分 类 号：S64[农业科学—蔬菜学]