蛋白酶激活受体2对糖尿病小鼠结肠运动的影响  

Effect of protease activated receptor 2 on colonic motility in diabetes mice

作　　者：李宇佳 陆红丽[1] 黄旭[1] 许文燮[1] LI Yu-jia;LU Hong-li;HUANG Xu;XU Wen-xie(Department of Anatomy and Physiology,Shanghai Jiao Tong University College of Basic Medical Sciences,Shanghai 200025,China)

机构地区：[1]上海交通大学基础医学院解剖和生理学系

出　　处：《上海交通大学学报（医学版）》2019年第11期1255-1260,共6页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金面上项目(31671192)~~

摘　　要：目的·观察蛋白酶激活受体2(protease activated receptor 2,PAR2)对糖尿病小鼠结肠运动的影响,并探究其作用机制。方法·通过腹腔注射链脲佐菌素构建小鼠1型糖尿病模型。分离小鼠结肠平滑肌和肠段,通过肌条张力收缩实验和结肠移行性复合运动实验,观察PAR2激动剂对结肠运动的作用,以及小电导钙激活钾通道(small conductance calcium-activated potassium channel,SK3通道)阻断剂对其作用的影响。结果·PAR2受体激动剂对糖尿病小鼠结肠平滑肌运动的抑制作用较正常小鼠明显增强,SK3通道阻断剂可以抑制PAR2受体激动剂的作用。结论·糖尿病小鼠结肠中PAR2功能亢进,PAR2可能通过SK3通道抑制结肠运动。Objective·To observe the effect of protease activated receptor 2(PAR2)on the colonic motility in diabetic mice and investigate the mechanism.Methods·The mouse model of type 1 diabetes mellitus was established by intraperitoneal injection of streptozotocin.The smooth muscle strips and segments of colons were isolated.The effects of PAR2 agonist on colonic motility were observed by muscle strip tension contraction and colonic migrating motor complex experiments.The effect of small conductance calcium-activated potassium channel(SK3 channel)antagonist on it was also observed.Results·PAR2 agonist inhibited colonic motility and colonic smooth muscle was more sensitive to PAR2 agonist in diabetic mice.PAR2 agonist-induced inhibition was inhibited by SK3 channel antagonist.Conclusion·PAR2 activity in diabetic mice colons is significantly enhanced,which may inhibit colonic motility through SK3 channel.

关 键 词：糖尿病 蛋白酶激活受体2 血小板衍生生长因子受体α阳性细胞 结肠运动 小电导钙激活钾通道 

分 类 号：R587.1[医药卫生—内分泌] R333.3[医药卫生—内科学]