共载阿霉素与姜黄素纳米粒的制备及其抗耐药活性研究  

Research on Preparation of Nanoparticles Loaded with Doxorubicin and Curcumin and Their Anti-tumor Activity in Vitro on Drug Resistance of Tumor Cells

作　　者：王未来 丁勇兴[1,2] 闫若寒 WANG Wei-lai;DING Yong-xing;YAN Ruo-han(Deperment of Pharmacy,The Third People's Hospital of Bengbu,Bengbu233010 China;Benbu City Hospital of Traditional Chinese Medicine,Bengbu233010 China)

机构地区：[1]蚌埠市第三人民医院药学部,安徽蚌埠233000 [2]蚌埠市第三人民医院普外科普外科,安徽蚌埠233000 [3]蚌埠市中医院药学部,安徽蚌埠233010

出　　处：《内蒙古医学杂志》2019年第11期1284-1286,1276,共3页Inner Mongolia Medical Journal

基　　金：蚌埠市科技计划项目(20150309)

摘　　要：目的肿瘤细胞的耐药性严重限制了化疗药物的临床应用,本研究成果表明,将化疗药物“敏感剂”与化疗药物联合应用可以有效地增加化疗药物的抗肿瘤活性。方法构建同时负载阿霉素(DOX)与化疗药物“敏感剂”姜黄素(Cur)的纳米递药系统,采用Zeta电位及纳米粒度分析仪、紫外-可见分光光度计及激光共聚焦显微镜等对纳米粒的形态、体外释药、体外抗肿瘤活性及纳米粒转运DOX在肿瘤细胞的分布等方面进行考察和评价。结果制得纳米粒的粒径为(188±12)nm,DOX与Cur的载药量分别为5%和10%,纳米粒的体外药物释放呈现pH依赖性,细胞实验结果显示Cur可以有效地增加DOX在耐药肿瘤细胞内的分布,因此显示出更高的细胞毒性。结论化疗药物“敏感剂”与化疗药物的联合应用对耐药肿瘤细胞有较好的疗效,具有进一步研究的价值。Objective Drug resistance of tumor cells severely limits the clinical use of chemo-therapeutic drugs.A combinational therapy consisting of chemo-sensitizer drugs and chemotherapy drugs could increase the antitumor activity of chemotherapeutic agents.Methods In this study,we developed doxorubicin(DOX)and curcumin(Cur)co-delivery nanoparticle,then used the zeta potential and particle size analyzer,UV-visible spectrophotometer and laser confocal microscope to investigate and evaluate the morphology of the nanoparticles in vitro drug release and in vitro anti-tumor active transport of DOX of tumor cells.Results We successfully made the nanoparticle with the average size of(188±12)nm and the drug loading of DOX and Cur 5%and 10%.Drug release of nanoparticles in vitro is pH-dependent.The cell experimental results show that Cur can increase the distribution of DOX in drug resistant tumor cells,thus showing higher cytotoxicity.Conclusion The combination of chemo-sensitizer drugs and chemotherapy drugs has certain curative effect on drug-resistant tumor cells,and has further research value.

关 键 词：肿瘤细胞耐药 化疗药物“敏感剂” 阿霉素 姜黄素 

分 类 号：R915[医药卫生—微生物与生化药学]