妊娠对肺高压大鼠肺动脉环张力及肺小动脉影响的实验研究  

Experimental study on the effects of pregnancy on the tension of pulmonary artery rings and pulmonary arterioles in rats with pulmonary arterial hypertension

作　　者：孙雨薇 朱琛 景赫[1] 闫莉[4] 张京岚[2] 赵明明 卢家凯[1] SUN Yuwei;ZHU Chen;JING He;YAN Li;ZHANG Jinglan;ZHAO Mingming;LU Jiakai(Department of Anesthesiology,Beijing Anzhen Hospital,Capital Medical University,Beijing Institute of Heart,Lung and Blood Vessel Diseases,Beijing 100029,China;不详)

机构地区：[1]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所麻醉科,100029 [2]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所综合外科监护,100029 [3]首都医科大学附属北京友谊医院麻醉科 [4]中国医学科学院基础医学研究所北京协和医学院基础学院病理生理学系

出　　处：《心肺血管病杂志》2019年第11期1178-1183,共6页Journal of Cardiovascular and Pulmonary Diseases

摘　　要：目的:通过观察大鼠肺动脉环张力及肺小动脉形态学变化,探讨肺动脉高压发病机理及妊娠对肺动脉高压疾病进展影响的潜在机制。方法:雌性,6周龄,SD大鼠55只,随机分为四组:空白对照组(n=10);野百合碱组(MCT,n=13);妊娠组(n=12);妊娠MCT组(n=20)。动物模型[1]完成后经颈静脉置管及股动脉置管采集血流动力学数据,并取大鼠三级肺动脉环,观察ACh对各组大鼠肺动脉环舒张作用;同时采集肺组织进行HE染色,观察肺小动脉损伤情况。结果:与MCT相比,妊娠MCT组MPAP显著降低(P<0.05);MCT组ACh诱导的内皮依赖性动脉舒张率比空白对照组显著降低(P<0.05);妊娠MCT组ACh诱导的内皮依赖性动脉舒张率比妊娠组及MCT组均显著降低(P<0.05);妊娠组与空白对照组大鼠肺血管内皮依赖性舒张功能无显著性差异(P>0.05);MCT组、妊娠MCT组大鼠肺动脉壁及肺小动脉弹性中膜厚度增加,内径逐渐变窄,肺小动脉有微血管瘤形成,部分小血管明显闭塞;妊娠MCT组较MCT组增厚肺小动脉密度明显增加(P<0.05)。结论:妊娠会降低肺高压大鼠肺动脉内皮依赖性舒张功能,促进肺小动脉损伤,加重肺动脉高压。Objective:To observe the changes in the tension of pulmonary artery rings and the morphology of pulmonary arterioles and discuss the pathogenesis of pulmonary arterial hypertension and the effect of pregnancy on pulmonary arterial hypertension.Methods:55 female 6-week-old SD rats were randomly divided into 4 groups:Blank control group,(n=10),monocrotaline(MCT)group(n=13),pregnancy group(n=12)and pregnancy MCT group(n=20).After successful modeling,hemodynamic data were collected by internal jugular catheterization and femoral artery catheterization.Subsequently,the vasodilatory effects of ACh were studied on rat pulmonary artery tertiary branches rings in all groups.At the same time,lung tissue specimens were collected to observe the damage to pulmonary arterioles.Results:Compared with MCT group,MPAP was significantly decreased in pregnant MCT group(P<0.05);the Ach-induced endothelium-dependent arterial diastole rate in the MCT group was significantly lower than that in the blank control group(P<0.05),and the Ach-induced endothelium-dependent arterial diastole rate in the pregnant MCT group was significantly lower than that in the pregnant group or MCT group(P<0.05).There was no significant difference in pulmonary endothelium-dependent diastolic function between the pregnant group and the blank control group(P>0.05).In the MCT group and the pregnant MCT group,the thickness of the rats’pulmonary artery wall and pulmonary arterioles increased,the inner diameter gradually narrowed,hemangioma capillanisum was formed in the pulmonary arterioles,and some small blood vessels were obviously occluded,Compared with MCT group,thicked arterial density was significant increased(P<0.05).Conclusions:Pregnancy further reduces pulmonary endothelium-dependent diastolic function of PAH rats and promotes the damage to pulmonary arterioles.

关 键 词：肺动脉高压 妊娠 内皮依赖性舒张功能 肺小动脉损伤 大鼠 

分 类 号：R54[医药卫生—心血管疾病]