HPLC法测定人血浆中莫西沙星浓度及其在MDR-TB治疗中的临床应用  

Determination of Moxifloxacin in Plasma by HPLC and Clinical Application in Treatment of MDR-TB

作　　者：张婷婷[1] 卢弢 徐然 ZHANG Ting-ting;LU Tao;XU Ran(Department of Pharmacy,The Second Hospital of Nanjing,Nanjing University of Chinese Medicine,Nanjing Public Health Center,Nanjing 210003,China;Nanjing Institute for Food and Drug Control,Nanjing 211100,China)

机构地区：[1]南京中医药大学附属药学部南京医院药学部南京市第二医院南京市公共卫生医疗中心,江苏南京210003 [2]南京市食品药品监督检验院,江苏南京211100

出　　处：《海峡药学》2019年第11期62-65,共4页Strait Pharmaceutical Journal

摘　　要：目的建立高效液相色谱方法测定血浆中莫西沙星的浓度,为耐多药结核患者中应用莫西沙星的临床安全合理用药提供参考。方法人血浆经甲醇沉淀蛋白后,采用高效液相-紫外色谱法测定。色谱柱为岛津Inertsustain C 18柱,流动相为甲醇-醋酸盐缓冲液(38∶62),流速为1.0mL·min-1,柱温为45℃,检测波长为295nm,进样体积为20μL。结果莫西沙星的保留时间约为11.3min,血浆内源物质对测定无干扰;莫西沙星血药浓度在0.1~10.0μg·mL-1范围内线性关系良好;最低定量限浓度为0.1μg·mL-1;绝对回收率为78.02%~84.09%,相对回收率为80.01%~111.11%,日内、日间精密度的RSD均<16.06%。结论该测定方法具有操作简单,回收率高,结果准确等优点,可以为临床合理安全用药提供依据。OBJECTIVE To establish the method for the determination of plasma concentration of moxifloxacin,to further evaluate the clinical safety of the MDR-TB patients treated with moxifloxacin.METHODS Normal plasm a was pretreated with methanol for protein precipitation and determined by HPLC-UV;Column:Inertsustain C 18 column(4.6mm×250mm,5μm);Mobile phase:methanol-buffered solution(38∶62),the buffer solution was an acetic acid-sodium acetate buffer solution with pH 4.70;Flow rate:1.0mL·min-1;Column temperature:45℃;Detection wavelength was 295nm;Injection volume:20μL.RESULTS The retention time of moxifloxacin was 11.3min,which showed a good shape,and was not interfered with the determination by plasma endogenous peak.The plasma concentration ranged from 0.1 to 10μg·mL-1.The minimum quantitative concentration was 0.1μg·mL-1,the absolute recoveries were 78.02%-84.09%,and relative recoveries were 80.01%-111.11%,and RSDs of intra-day and inter-day were less than 16.06%.CONCLUSIONThe method is simple and high recovery rate of extraction and high accura cy.It could be the reference of the clinical safety medication.

关 键 词：高效液相色谱法 莫西沙星 血药浓度 耐多药肺结核 用药安全 

分 类 号：R969.1[医药卫生—药理学]