病毒灭活疫苗诱导云斑尖塘鳢脾脏转录组分析  

Transcriptome Analysis of Spleen Induced by Inactivated Viral Vaccine in Marbled Sand Goby, Oxyeleotris marmoratus

作　　者：周阳 李本旺[3] 林强 付小哲[1] 李敏[3] 梁红茹 刘礼辉[1] 卢伟华[3] 李宁求 Zhou Yang;Li Benwang;Lin Qiang;Fu Xiaozhe;Li Min;Liang Hongru;Liu Lihui;Lu Weihua;Li Ningqiu(Key Lab of Aquatic Animal Immune Technology of Guangdong Province,Key Lab of Fishery Drug Developmen of Ministry of Agriculture,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou,510380;College of Fishery and Life Science,Shanghai Ocean University,Shanghai,201306;Aquatic Animal Epidemic Prevention and Quarantine Station in Dongguan,Dongguan,523127)

机构地区：[1]中国水产科学研究院珠江水产研究所,农业部渔用药物创制重点实验室,广东省水产动物免疫技术重点实验室,广州510380 [2]上海海洋大学水产与生命学院,上海201306 [3]广东省东莞市水生动物防疫检疫站,东莞523127

出　　处：《基因组学与应用生物学》2019年第10期4404-4412,共9页Genomics and Applied Biology

基　　金：广东省渔港建设和渔业产业发展专项(粤财农(2017)17号);东莞市市级财政资金项目共同资助

摘　　要：为阐明云斑尖塘鳢对病毒灭活疫苗的免疫应答机制,本研究采用RNA-sequencing技术结合生物信息学方法分析了传染性脾肾坏死病毒、鳜弹状病毒二联灭活疫苗接种云斑尖塘鳢后脾脏转录谱。分别构建疫苗接种后7 d、14 d脾脏cDNA文库并对其进行高通量测序,共获得85547条Unigenes,其中32386条Unigenes被注释;共筛选得到13593个差异表达基因,其中免疫后7 d上调基因1291个、下调基因2030个,14 d上调基因3732个、下调基因6540个。GO功能注释显示,差异表达基因编码蛋白主要与免疫应答、细胞黏附以及细胞增殖相关;KEGG分析表明差异表达基因集中在细胞-细胞受体互作通路、细胞粘附分子通路和MAPK信号通路等。选取差异表达的IL-1β、IL-12、MHCⅠ、MHCⅡ等4个基因进行qPCR验证,结果与转录谱结果相符。研究结果为阐明云斑尖塘鳢对传染性脾肾坏死病毒、鳜弹状病毒二联灭活疫苗的免疫应答机制提供依据。In order to elucidate the mechanism of immune response against the inactivated viral vaccine,the transcriptome of spleen induced by infectious spleen and kidney necrosis virus and rhabdovirus inactivated vaccine were determined and analyzed by RNA-sequencing and bioinformatics analysis.In the samples harvested at 7 d and 14 d post of immunization,a total of 85547 unigenes were obtained,32386 unigenes were annotated,13593 genes were differentially expressed in spleen of vaccination groups compared to control,including 1291 or 2030 up-regulated genes and 3732 or 6540 down-regulated genes.GO annotation showed that the differentially expressed genes encoded proteins were mainly related to immune response,cell adhesion and cell proliferation.KEGG analysis showed that the differentially expressed genes focused on cell-cell receptor interaction pathway,cell adhesion molecule pathway and MAPK signaling pathway.Differentially expressed IL-1β,IL-12,MHCⅠ,MHCⅡwere selected and verified by qPCR.The results were consistent with the results of transcriptional profiling.This study provided the basis for further elucidation of the mechanism of immune response against infectious spleen and kidney necrosis virus and rhabdovirus dual inactivated vaccine.

关 键 词：云斑尖塘鳢 转录组 传染性脾肾坏死病毒 鳜弹状病毒 免疫应答 

分 类 号：S85[农业科学—兽医学]