盐胁迫下白蜡无性系苗期的耐盐性综合评价  

Comprehensive evaluation of salt tolerance of clones of Fraxinusin spp. seedling stage under salt stress

作　　者：闫文华 吴德军[1] 燕丽萍[1] 王因花[1] 任飞[1] 燕琳 刘婕 于林霖 Yan Wenhua;Wu Dejun;Yan Liping;Wang Yinhua;Ren Fei;Yan Lin;Liu Jie;Yu Linlin(Shandong Provincial Academy of Forestry,Shandong Provincial Key Laboratory of Forest Tree Genetic Improvement,Jinan 250014,Shandong,China;College of Forestry,Shandong Agricultural University,Taian 271000,Shandong,China;Haiyang Zhaohu Mountain Provincial Nature Reserve Management Office,Haiyang 265100,Shandong,China;Forestry Bureau in Haiyang City,Haiyang 265100,Shandong,China)

机构地区：[1]山东省林业科学研究院,山东省林木遗传改良重点实验室,山东济南250014 [2]山东农业大学林学院,山东泰安271000 [3]海阳市招虎山省级自然保护区管理处,山东海阳265100 [4]海阳市林业局,山东海阳265135

出　　处：《北京林业大学学报》2019年第11期44-53,共10页Journal of Beijing Forestry University

基　　金：山东省农业良种工程项目（2019LZGC009）;山东省重点研发计划项目（2019GNC106143）;林业知识产权转化运用项目（知2017-9）

摘　　要：【目的】本研究通过对9个白蜡无性系的耐盐性进行综合评价,为探究白蜡无性系的耐盐能力,选育优良耐盐林木品种,利用生物措施提高土地生产力提供理论依据。【方法】以1年生白蜡无性系扦插盆栽苗为试验材料,对9个无性系在不同盐胁迫条件下(0、2、4、6、8 g/L)的生长与生理指标的变化进行研究。【结果】在盐胁迫下,各无性系的生长受到明显抑制,并且随着NaCl质量浓度增大,无性系的生长指标(苗高增量、地径增量、生物量累积)都呈现下降的趋势,无性系YL的下降幅度最为平缓;9个无性系中的叶绿素含量也随着盐质量浓度升高而下降,YL与其他无性系存在显著性差异(P<0.05)。盐胁迫导致无性系体内细胞膜透性与MDA含量增大,但在同一盐质量浓度时,无性系YL与Y3低于其他无性系;当盐胁迫逐步增大时,脯氨酸、可溶性糖含量因无性系品种不同而呈现不同的趋势,其中在YL、L5、QB、L2、Y3无性系中一直处于增长状态,在其余4种无性系中呈现单峰曲线,最高值出现在盐质量浓度为6 g/L的处理中;SOD酶活性呈现先升高后下降的趋势,无性系YL酶活性最高,与其他无性系有显著性差异(P<0.05)。【结论】本试验采用主成分分析法对9个白蜡无性系的耐盐性进行综合评价,耐盐能力大小依次为YL、Y3、L5、QB、JJ、JN11、HX、L2、J10,试验结果为白蜡耐盐无性系的选育提供理论基础和育种材料。[Objective]This paper aims to explore the salt tolerance of ash tree clones,breed excellent salt tolerant of clones,and improve land productivity through biological measures.[Method]Cutting potted seedlings of 1-year-old ash clones were taken as materials,and their growth and physiological index of 9 clones under different salt stress conditions(0,2,4,6,8 g/L)were studied.[Result]Under salt stress,the growth of clones was significantly inhibited,and with the increase of NaCl concentration,the growth indicators(seedling height increment,ground diameter increment,biomass accumulation)of clones showed a decreasing trend,and the decline of‘YL’of clones was the slowest;the chlorophyll content of nine clones also decreased with the increase of salt concentration,and there was significant difference between saline wax and other clones(P<0.05).Under salt stress,the cell membrane permeability and MDA content of clones increased,but at the same salt concentration,‘YL’and‘Y3’of clones were lower than those of other clones;when salt stress increased gradually,the contents of proline and soluble sugar showed different trends according to different varieties.Among them,osmotic regulators in‘YL’,‘L5’,‘QB’,‘L2’and‘Y3’had been increasing,the content of the other four clones showed a single peak curve,and the highest value reached 6 g/L.SOD activity increased first and then decreased.Its value of‘YL’was the highest,which was significantly different from that of other clones(P<0.05).[Conclusion]Principal component analysis was used to evaluate the salt tolerance of nine ash tree clones.The salt tolerance of nine ash tree clones was ordered as follows:‘YL’,‘Y3’,L5’,QB,JJ,JN11,HX,‘L2’and‘J10’.The results provided are not only theoretical basis but also breeding materials for the breeding of ash tree salt tolerant clones.

关 键 词：白蜡 无性系 盐胁迫 耐盐性 

分 类 号：S728.5[农业科学—林木遗传育种]