年龄和性别因素对健康成人面部皮肤细菌和真菌的影响  

Effect of age and gender on bacteria and fungi on the healthy face of adults

作　　者：钟彩梅 邓裕华 周美凤[1] 赵伟峰[1] Zhong Caimei;Deng Yuhua;Zhou Meifeng;Zhao Weifeng(Department of Dermatology,Center for Prevention and Treatment of Chronic Diseases of Shunde,Foshan 528399,Guangdong,China;Department of Pathogen Biology and Experimental Teaching Centre of Preventive Medicine,School of Public Health,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]广东省佛山市顺德区慢性病防治中心皮肤科,528399 [2]南方医科大学公共卫生学院预防医学实验教学中心,广州510515

出　　处：《中华皮肤科杂志》2019年第12期889-898,共10页Chinese Journal of Dermatology

基　　金：佛山市卫生和计生局医学科研课题(20180353)。

摘　　要：目的通过宏基因组测序分析年龄及性别对健康面部皮肤微生物群落的影响。方法2017年6月至2018年6月在广东省佛山市顺德区慢性病防治中心皮肤科门诊招募36例面部皮肤健康志愿者,包括16例年轻志愿者(男9例,女7例)和20例老年志愿者(男女各10例);年轻组年龄24~31(27.1±1.3)岁,老年组年龄61~84(75.8±2.2)岁。采集志愿者面颊皮肤微生物样品,提取样品DNA,并进行宏基因组测序及生物信息学分析,分析年龄及性别因素对面部皮肤微生物群落的影响。统计分析采用两独立样本t检验、Wilcoxon秩和检验及Pearson相关分析。结果老年组面部皮肤细菌和真菌群落的α多样性(Shannon指数0.98±0.07、1.11±0.05)均显著高于年轻组(0.72±0.09、0.81±0.05;t=2.201、3.836,P=0.035、<0.001)。主成分分析显示,年龄也能显著影响面部皮肤细菌和真菌群落的β多样性(t=6.991、11.591,均P<0.001)。男性组与女性组间细菌(Shannon指数:0.83±0.08比0.92±0.09)和真菌群落的α多样性(0.92±0.06比1.04±0.05)差异均无统计学意义(t=0.801、1.332,均P>0.05)。性别因素仅影响细菌群落的β多样性(t=2.149,P=0.020),而不影响真菌群落的β多样性(t=0.439,P=0.663)。此外,老年组细菌(t=1.995,P=0.020)和真菌(t=2.464,P=0.025)代谢途径的活跃程度均低于年轻组,而性别因素则未影响皮肤细菌(t=0.895,P=0.378)和真菌(t=0.483,P=0.631)代谢途径的活跃程度。根据老年组与年轻组间有差异的细菌和真菌的相对丰度计算两者之间的相关性,结果显示,细菌-真菌、细菌-细菌和真菌-真菌之间存在正相关或负相关关系。结论年龄因素而非性别因素显著地影响健康面部皮肤细菌和真菌群落的α和β多样性及其代谢途径。Objective To evaluate the effect of age and gender on skin microbiome on the face of healthy adults by metagenomic sequencing.Methods From June 2017 to June 2018,36 adult volunteers with healthy facial skin were enrolled from the Department of Dermatology,Center for Prevention and Treatment of Chronic Diseases of Shunde,including 16 young volunteers(9 males and 7 females)aged 24-31(27.1±1.3)years and 20 senior volunteers(10 males and 10 females)aged 61-84(75.8±2.2)years.Skin microbe samples were obtained from the cheek of volunteers,and DNA was extracted from these samples and subjected to metagenomic sequencing and bioinformatic analysis,so as to evaluate the effect of age and gender factors on microbiota on the healthy facial skin of adults.Statistical analysis was carried out by using two independent-sample t test,Wilcoxon rank sum test and Pearson correlation analysis.Results The senior group showed significantly higher α diversities of bacterial and fungal communities on the facial skin(Shannon index:0.98±0.07,1.11±0.05 respectively)compared with those in the young group(0.72±0.09,0.81±0.05 respectively;t=2.201,3.836,P=0.035,<0.001 respectively).Principal component analysis revealed that age could significantly affect β diversities of bacterial and fungal communities on the facial skin(t=6.991,11.591 respectively,both P<0.001).There were no significant differences inαdiversities of bacterial and fungal communities between males and females(Shannon index:bacteria,0.83±0.08 vs.0.92±0.09;fungi,0.92±0.06 vs.1.04±0.05;t=0.801,1.332 respectively,both P>0.05).Gender factor could only affect theβdiversity of bacterial communities(t=2.149,P=0.020),but not the β diversity of fungal communities(t=0.439,P=0.663).Moreover,the activity of metabolic pathways in bacterial and fungal communities was significantly lower in the senior group than in the young group(t=1.995,2.464,P=0.020,0.025,respectively),while gender factor did not affect the activity of metabolic pathways in bacterial and fungal communities(t=

关 键 词：皮肤 微生物群落 细菌 真菌 年龄因素 性别因素 宏基因组学 

分 类 号：R44[医药卫生—诊断学]