人绒毛膜来源间充质干细胞体外成血管能力探讨  

Angiogenic Potential of Human Chorion-Derived Mesenchymal Stem Cells in Vitro

作　　者：钱锦[1] 彭海林[1] 黄颖[1] QIAN Jin;PENG Hai-lin;HUANG Ying(Department of Clinical Laboratories,Taizhou People’s Hospital,Taizhou 225300,China)

机构地区：[1]泰州市人民医院检验科

出　　处：《南昌大学学报（医学版）》2019年第5期6-11,共6页Journal of Nanchang University：Medical Sciences

基　　金：泰州市人民医院院级科研基金(ZL201615)

摘　　要：目的探讨体外分离培养人绒毛膜来源间充质干细胞的方法,评价诱导的间充质干细胞分化为血管内皮细胞及体外成血管的可行性。方法从人胎盘绒毛膜组织中分离间充质干细胞及培养,采用流式细胞术检测细胞表面抗原表达;培养第2代细胞进行血管内皮细胞分化诱导,2周后采用免疫荧光染色法鉴定Matrigel基质胶中CD31和vWF的表达。结果从人胎盘绒毛膜组织中分离培养的细胞表达CD73、CD90和CD105,不表达CD34和HLA-DR。人绒毛膜间充质干细胞向血管内皮细胞定向诱导2周后,形态由长梭状逐步变为圆形、铺路石状,并在Matrigel基质胶上培养12 h后有管腔样结构形成,免疫荧光染色显示管腔样结构的细胞表达血管内皮细胞表面标记物CD31和vWF因子。结论胎盘绒毛膜可用于分离培养间充质干细胞,培养的细胞具有诱导分化为血管内皮细胞的潜能。Objective To explore the methods to isolate and culture human chorion-derived mesenchymal stem cells(MSCs),and to evaluate the feasibility of differentiation of MSCs into vascular endothelial cells in vitro.Methods MSCs were isolated from human placental chorionic tissue and cultured in vitro.Cell surface antigen expression was detected by flow cytometry.The cells in second passage were used for induction of endothelial differentiation.After 2 weeks,the expression of CD31 and vWF in Matrigel was identified by immunofluorescence staining.Results The human chorion-derived MSCs expressed CD73,CD90 and CD105,but did not express CD34 and HLA-DR.After MSCs were induced for 2 weeks,the cells gradually changed from fusiform shape to circle or paving stone shape.Furthermore,a lumen-like structure was formed after 12 hours of culture on Matrigel.Immunofluorescence staining showed that cells in the lumen-like structure expressed vascular endothelial cell surface markers CD31 and vWF.Conclusion Placental chorion can be used to separate MSCs,and the cultured cells have the potential to differentiate into vascular endothelial cells.

关 键 词：绒毛膜 间充质干细胞 血管内皮细胞 

分 类 号：R34[医药卫生—基础医学]