地锦草与5种抗菌药联用对猪链球菌的体外抑菌效果  

Antibacterial Effect of Euphorbia humifusa Combining with Five Antimicrobials against Streptococcus suis in vitro

作　　者：江超 姚飞虹 张之怡 程庆 罗稀楷 李嗣忻 舒宇 王歧本 JIANG-Chao;YAO Fei-hong;ZHANG Zhi-yi(The Basic Medical College of Xiangnan University,Chenzhou,Hunan 423000;Pharmacy College of Xiangnan University,Chenzhou,Hunan 423000)

机构地区：[1]湘南学院基础医学院,湖南郴州423000 [2]湘南学院药学院,湖南郴州423000

出　　处：《安徽农业科学》2019年第24期114-116,共3页Journal of Anhui Agricultural Sciences

基　　金：湖南省教育厅科研项目(2015C1286,2016C1492);湘南学院2018学年校级实验室开放项目(27);湖南省普通高等学校重点实验室湘南学院心脑血管天然药物研究实验室项目(XN2017K05)

摘　　要：[目的]探究地锦草与其他抗菌药物对猪链球菌的体外联合抗菌效果,达到有效防止猪链球菌的目的。[方法]采用琼脂稀释法、微量肉汤棋盘稀释法,分别测定了地锦草与庆大霉素、青霉素、头孢曲松、阿莫西林、硫酸链霉素对3株猪链球菌的最小抑菌浓度(MIC)及分级抑菌浓度指数(FIC)。[结果]地锦草和5种抗菌药联用后对3株链球菌的MIC有不同程度的降低,联用后都表现为相加或协同效果。[结论]地锦草在体外与5种抗菌药联用可减少抗菌药物用量,增强药物对细菌的抑制作用。[Objective]In order to effectively prevent Streptococcus suis,Euphorbia humifusa combining with five antimicrobials against S.suis in vitro was studied.[Method]The classical agar dilution method and micro broth checkerboard dilution method were used,the sensitivity effects of Euphorbia humifusa combining with gentamicin,streptomycin sulfate,penicillin,ceftriaxone,amoxicillin againsts three serotypes S.suis were tested in vitro,the minium inhibitor concentration(MIC)and the fractional inhibitory concentration(FIC)were calculated.[Result]After combining,the MIC of the Euphorbia humifusa and the five kinds of antibacterials to the three strains of S.suis had different degrees of reduction,the combined effect was additive or synergistic.[Conclusion]It is suggested that the combination of five kinds of antibacterial with Euphorbia humifusa in vitro can reduce the antibacterial drugs and enhance the inhibitory effect of drugs on bacteria.

关 键 词：地锦草 抗菌药物 体外联合药敏试验 猪链球菌 

分 类 号：S853.7[农业科学—临床兽医学]