抗根肿病大白菜近等基因系的构建  

Construction of Near-isogenic Lines of Chinese Cabbage with Clubroot Resistance

作　　者：张红[1] 黄志银[1] 李梅[1] 范伟强 温娟娟 王超楠[1] ZHANG Hong;HUANG Zhi-yin;LI Mei(Tianjin Kerun Vegetable Research Institute,National Key Laboratory of Vegetable Germplasm Innovation,Tianjin Key Laboratory of Vegetable Genetic and Breeding Enterprises,Tianjin 300381)

机构地区：[1]天津科润蔬菜研究所,蔬菜种质创新国家重点实验室,天津市蔬菜遗传育种企业重点实验室,天津300381 [2]天津师范大学生命科学学院,天津300387

出　　处：《安徽农业科学》2019年第24期128-132,共5页Journal of Anhui Agricultural Sciences

基　　金：青年科研人员创新研究与实验项目(2018013,2018003);天津市现代农业产业技术体系创新团队建设专项计划(ITTVRS2017003)

摘　　要：为培育具有抗根肿病基因的青麻叶大白菜,丰富天津地方种质资源,以优良的青麻叶自交系H227作为轮回亲本,12G57自交系作为抗根肿病基因的供体亲本,利用分子标记辅助选择技术,在BC 3F 2世代即选育出4株轮回亲本基因组含量为100%的纯合抗性单株,快速高效地构建了抗根肿的青麻叶近等基因系。从抗病性、农艺性状等方面分别对所获得4个近等基因系进行评价,结果发现表型均达抗病,主要农艺性状也均与轮回亲本H227无显著差异,表明今后不仅可用作青麻叶抗病育种实践的材料,而且可作为根肿病抗病基因定位的最佳材料。In order to cultivate abutilon leaves Chinese cabbage with clubroot resistance gene and enrich local germplasm resources in Tianjin,taking excellent inbred lines H227 abutilon leave as recurrent parents,12G57 inbred lines as the donor parents of clubroot resistance gene,four homozygous resistant plants with 100%genome content of recurrent parents were bred in BC 3F 2 generation,and the near-isogenic lines with resistance to clubroot disease were constructed rapidly and efficiently by using marker-assisted selection technology.From the aspects of disease resistance,agronomic characters of four near-isogenic lines,all the phenotypes were disease-resistant,and the main agronomic traits were not significantly different from the recurrent parent H227,indicating that it could not only be used as the material for disease resistance breeding of green leaves in the future,but also as the best material for the location of clubroot resistance gene.

关 键 词：青麻叶大白菜 抗根肿病基因 近等基因系 分子标记辅助选择 

分 类 号：S634.1[农业科学—蔬菜学]