不同PRRS疫苗组合对仔猪免疫效果的研究  

Study on Immune Effect of Different PRRS Vaccine Combinations on Piglets

作　　者：蔡汝健 蒋智勇 勾红潮 楚品品 李艳 宋帅 徐志宏 李春玲 CAI Rujian;JIANG Zhiyong;GOU Hongchao;CHU Pinpin;LI Yan;SONG Shuai;XU Zhihong;LI Chunling(Institute of Animal Health,Guangdong Academy of Agricultural Sciences/Key Laboratory of Livestock Disease Prevention of Guangdong Province/Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province,Ministry of Agriculture and Rural Areas,Guangzhou 510640,China)

机构地区：[1]广东省农业科学院动物卫生研究所/广东省畜禽疫病防治研究重点实验室/农业农村部兽用药物与诊断技术广东科学观测实验站

出　　处：《广东农业科学》2019年第10期93-98,共6页Guangdong Agricultural Sciences

基　　金：国家重点研发计划项目(2018YFD0500804);广东省公益研究与能力建设专项资金(农业科技领域,2017B020202002);广东省科技创新战略专项(2018KJYZ007)

摘　　要：【目的】针对猪繁殖与呼吸综合征(Porcine Reproductive and Respiratory Syndrome,PRRS)毒株存在多样性和疫苗免疫效果不佳的问题,探索PRRS疫苗对仔猪最佳免疫方案。【方法】分别在A、B、C 3个猪场开展试验,每个猪场采用4种不同的PRRS疫苗组合:第1种,14日龄同时免疫PRRS弱毒苗和灭活苗;第2种,4日龄免疫PRRS弱毒苗和35日龄免疫灭活苗;第3种,14日龄和35日龄免疫弱毒苗;第4种,14日龄免疫弱毒苗。通过免疫后的抗体滴度、病毒血症和生产参数评估PRRS疫苗免疫效果。【结果】不同免疫组合比较发现,A2、B3、C3抗体水平最高,S/P值分别为2.50、2.63和2.99;A2、B2、B3、C1血清中PRRSV核酸阳性率最低,分别为12.5%、0、0、6.3%;A1、B2、C1发病率最低,分别为10%、2%和8%。【结论】14日龄同时免疫PRRS弱毒苗和灭活苗,14日龄免疫PRRS弱毒苗和35日龄免疫灭活苗,两种免疫组合免疫效果最好。【Objective】For the problem of the diversity of PRRSV strain and the ineffectiveness of vaccine immunization,the best vaccination scheme for porcine reproductive and respiratory syndrome vaccine was explored.【Method】The study was carried out in three pig farms(A,B and C)and four different combinations of PRRS vaccines were used in each farm.Group 1:14-day-old piglets immunized with PRRS attenuated vaccine and inactivated vaccine.Group 2:14-day-old piglets immunized with PRRS attenuated vaccines and 35-day-old piglets immunized with inactivated vaccine.Group 3:14-day-old and 35-day-old piglets immunized with attenuated vaccines.Group 4:14-day-old piglets immunized with PRRS attenuated vaccines.The effects of the PRRS vaccine against PRRSV were evaluated through antibody titer,viremia,and production parameters.【Result】By comparing different immunization groups,it was found that A2,B3 and C3 had the highest antibody levels and the S/P were 2.50,2.63 and 2.99,respectively.The positive rates of PRRSV nucleic acid in serum A2,B2,B3 and C1 were the lowest,which were 12.5%,0,0,6.3%,respectively.The incidences of A1,B2 and C1 were the lowest,which were 10%,2%and 8%,respectively.【Conclusion】The combined immunization of 14-day-old PRRS attenuated vaccine and inactivated vaccine,and 14-day-old PRRS attenuated vaccine and 35-day-old inactivated vaccine showed the best immunization effects.

关 键 词：猪繁殖与呼吸综合征 免疫方案 抗体滴度 病毒血症 免疫效果 

分 类 号：S855.3[农业科学—临床兽医学]