黄芪注射液干预糖尿病肾病大鼠后肾脏足细胞的变化  

Changes of glomerular podocyte in diabetic nephropathy rats by Astragalus injection

作　　者：赵安菊[1] 王智黔[1] 黄平[1] Zhao Anju;Wang Zhiqian;Huang Ping(Department of Officer,Guizhou Provincial People’s Hospital.Guiyang 550002,Guizhou,China)

机构地区：[1]贵州省人民医院

出　　处：《贵州医药》2019年第11期1693-1695,共3页Guizhou Medical Journal

基　　金：贵州省科技联合基金项目(编号:黔科合SY[2012]3121号)

摘　　要：目的电镜下观察黄芪注射液干预糖尿病肾病(DN)大鼠后肾脏足细胞的变化,探讨黄芪注射液对糖尿病肾病肾脏足细胞病的影响。方法设对照组(C组)、糖尿病肾病组(DN组)及黄芪注射液干预组(H组),建立链脲佐菌素(STZ)诱导的DN大鼠模型,电镜观察各组肾小球足细胞的形态变化。结果 (1)C组肾小球基底膜均匀,足细胞可见初级足突、次级突起、相邻两个足细胞之间的次级突起相互交错穿插,形成栅栏状,紧贴于毛细血管基膜外面。(2)DN组肾小球足细胞可见基底膜厚薄不均,足突广泛融合,裂孔消失,融合的足突与GBM粘连,周围可见反应性微绒毛存在,(3)H组大鼠足细胞足突融合、内皮细胞肿胀病变程度较DN组有所减轻。结论黄芪注射液可改善DN大鼠肾脏足细胞病变程度,从而可能改善肾小球滤过功能,减轻尿蛋白,改善肾功能,对糖尿病肾病的防治有一定积极作用。Objective To observe the changes of glomerular podocyte in diabetic nephropathy(DN) rats by Astragalus injection under electron microscope.Methods Adult Sprague-Dawley male rats were divided into diabetic group(DN), control group(C) and huangqi injection meddle(H). The rat model of STZ-induced DN was established. To observed the changes of glomerular podocyte of every group rat under electron microscope. Results In C group, The glomerular basement membrane(GBM) evenly, podocyte foot process that primary, secondary process, secondary processes between two adjacent podocytes staggered interspersed, palisade formation, close to the outside of capillary basement membrane. DN group rats showed glomerular podocyte basement membrane uneven thickness, the foot processes were fusion, the hole disappeared. Podocyte and GBM adhesion and fusion, are seen the reactive tomentulose around.H group rats showed fusion foot process of podocyte, the degree of swelling of endothelial cells lesion than in DN group reduced. Conclusion Huangqi injection could protect glomerular podocyte, it might maintain the normal glomerular filtration function and reduce the urinary protein excretion rate to the rats of diabetic nephropathy.

关 键 词：糖尿病肾病 足细胞 Nephri 电镜 黄芪注射液 

分 类 号：R587.1[医药卫生—内分泌] R692[医药卫生—内科学]