花生荚果大小和重量相关性状的QTL定位分析  

QTL mapping for traits of pod size and weight in cultivated peanut(Arachis hypogaea L.)

作　　者：周小静[1] 雷永[1] 夏友霖[2] 漆燕[2] 晏立英[1] 任小平[1] 黄莉[1] 罗怀勇[1] 刘念[1] 陈伟刚[1] 陈玉宁[1] 廖伯寿[1] 姜慧芳[1] ZHOU Xiao-jing;LEI Yong;XIA You-lin;QI Yan;YAN Li-ying;REN Xiao-ping;HUANG Li;LUO Huai-yong;LIU Nian;CHEN Wei-gang;CHEN Yu-ning;LIAO Bo-shou;JIANG Hui-fang(Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Ge?netic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China;Nanchong Acad?emy of Agricultural Sciences,Nanchong 637000,China)

机构地区：[1]中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [2]四川省南充市农业科学研究所,四川南充637000

出　　处：《中国油料作物学报》2019年第6期869-877,共9页Chinese Journal of Oil Crop Sciences

基　　金：农作物种质资源保护项目(2019NWB033);国家农作物种质资源共享服务平台(NICGR2019-014);中央级公益性科研院所基本科研业务费专项(1610172016002)

摘　　要：荚果相关性状是花生产量构成的重要成分。为解析花生产量及产量相关性状的遗传基础,挖掘稳定存在的QTL,以荚果大小和重量存在显著差异的中花5号和ICGV 86699及以其为亲本衍生的包含166个株系的重组自交系群体为材料,对3个荚果相关性状中荚果长、荚果宽在5个环境,百果重在6个环境下进行性状考察,并结合群体的基因分型数据进行QTL定位分析。共检测到9个荚果长QTL、10个荚果宽QTL和12个百果重QTL。有10个QTL在多个环境被重复检测到,其中6个QTL在不同地点重复检测到,为稳定表达QTL,且稳定表达的QTL中5个(qPLB06.2、qPLB06.3、qPWB06.2、qHPWB04.3、qHPWB06.3)在至少1个环境中贡献率超过10%。共发现5个QTL簇,其中位于B06上的QTL簇Ⅳ和Ⅴ,均在多个环境下检测到稳定调控荚果长、荚果宽和百果重的QTL共定位,表明这些荚果相关性状具有明显的遗传相关性。Pod-related traits are important to peanut yield. To better understand genetic basis of peanut yield,QTL mapping for pod-related traits was conducted using 166 recombinant inbred lines. The lines were derived from parents Zhonghua 5 and ICGV 86699 which were significantly different in pod size and weight. Pod length and width in 5 environments and 100-pod weight in 6 environments were investigated for QTL mapping by population phenotyping and by genotyping. Nine pod-length QTLs, 10 pod-width QTLs and 12 100-pod-weight QTLs were detected respectively. Among them, 10 QTLs were repeatedly detected in multiple environments(including 6 stable QTLs detected in different locations). Among the 6 stable ones, 5 QTLs(qPLB06.2, qPLB06.3, qPWB06.2, qHPWB04.3 and qHPWB06.3) had more than 10% PVE(phenotypic variation explained) in at least one environment.Five QTL clusters were identified. QTL clusters Ⅳ and Ⅴ, located on B06, were collocated with pod-related QTLs,which were stably detected in multiple environments. The collocations showed obvious genetic correlations among the pod traits.

关 键 词：花生 荚果 QTL定位 稳定QTL QTL簇 

分 类 号：S565.2[农业科学—作物学]