油菜素内酯在不同生育期对两品系甘蓝型油菜的生长调控  

Epibrassinolide regulation on oilseed rape(Brassica napus L.) in different period

作　　者：冯韬[1,2] 谭晖 徐江林[1] 官春云 FENG Tao;TAN Hui;XU Jiang-lin;GUAN Chun-yun(College of Agronomy,Hunan Agricultural University/National Oilseed Crops Improvement Center in Hunan,Changsha 410128,China;College of Biological and Agricultural,Zunyi Normal University,Zunyi 563002,China)

机构地区：[1]湖南农业大学农学院/国家油料改良中心湖南分中心,湖南长沙410128 [2]遵义师范学院生物与农业科技学院,贵州遵义563002

出　　处：《中国油料作物学报》2019年第6期904-913,共10页Chinese Journal of Oil Crop Sciences

基　　金：国家科技支撑计划课题(2014BAD11B03);国家油菜产业技术体系(CARS-13)

摘　　要：为了解油菜素内酯对甘蓝型油菜品质及弱光反应的影响,以湘油15号的2个辐照突变品系(含油量和产油量明显不同的XY881和XY883)为材料,研究油菜素内酯(epibrassinolide, 2,4-BL)在不同生育阶段对甘蓝型油菜生长发育的影响。通过盆栽试验和2014-2016年两年度田间试验,分析2,4-BL对种子萌发、营养生长、产油量及相关构成因子的影响。结果表明,2,4-BL对两品系的影响具有相似规律,包括:对种子萌发具有剂量效应,低浓度下促进种子萌发,高浓度下抑制种子萌发;在全生育期低剂量的2,4-BL促进主茎伸长、提高光合效率、促进结实,但不影响成熟种子大小和含油量;在盛花后使用,延缓种子成熟而造成授粉后40 d种子含油量下降;长期短间隔使用2,4-BL降低调控作用。在分子水平上,2,4-BL诱导油菜素类固醇信号通路和光信号通路关键转录因子BnaPIF4、BnaBZR1和BnaBES的基因表达。推测2,4-BL通过诱导甘蓝型油菜中油菜素内酯和光信号途径相关转录因子基因表达,激活下游调控的基因网络,通过促进光合作用延长营养生长时间、促进主茎生长和结实,进而影响产油量。因此认为油菜素内酯可以作为生长调节剂在油菜生产中使用,建议低浓度或长间隔施用,且尽量在油菜盛花期之前使用。To understand the effect of brassinolide on quality and dim light reaction of Brassica napus L., 2 irradiated mutants(XY881 and XY883) of B. napus cv Xiangyou 15 were investigated to study the effects of epibrassinolide(2,4-BL) on seed germination, vegetative growth, oil production and related components by pot experiment and field trials from 2014 to 2016. Results showed that 2,4-BL had the same effects on both lines. 2,4-BL had dose effect on seed germination. Low concentration promoted seed germination. Low 2,4-BL promoted elongation of main stem, increased photosynthetic efficiency, and promoted fruiting without affecting seed size and seed oil. Using 2,4-BL after flowering delayed seeds ripen and led to lower oil content. Regulation of 2,4-BL decreased in the case of long-term continuous use. Gene expression data showed that 2,4-BL induced(BnaPIF4, BnaBZR1 and BnaBES)gene expression of the key transcription factors in brassinosteroid signalling pathway and the light signalling pathway. It suggested that 2,4-BL activated downstream regulatory gene network to promote vegetative growth and silique growth by promoting photosynthesis. Thus 2,4-BL could be used as growth regulator in rapeseed production,but be applied at low concentration or long intervals, with the best result before the full flowering stage.

关 键 词：油菜素内酯 甘蓝型油菜 光合作用 种子产油量 基因表达 

分 类 号：S565.4[农业科学—作物学]