CD80、TNF-a和IL-4在子痫前期中的表达及意义  

Expression of CD80, TNF-α and IL-4 in preeclampsia and its value on clinical prognosis

作　　者：王莉[1,2] 丁依玲[1] WANG LI;DING Yi-ling(Department of Obstetrics, Second Xiangya Hospital of Central South University , Hunan Changsha 410001,China;Department of Obstetrics, Hainan Provincial People's Hospital, Hainan Haikou 570000, China)

机构地区：[1]中南大学湘雅二医院产科,湖南长沙410001 [2]海南省人民医院产科,海南海口570013

出　　处：《海南医学院学报》2019年第23期1801-1805,共5页Journal of Hainan Medical University

基　　金：海南自然科学基金面上项目(817306)~~

摘　　要：目的观察子痫前期患者外周血CD80的表达,并探讨其与肿瘤坏死因子-a(TNF-a)、白细胞介素-4(IL-4)的相关性。方法选取2016年5月~2017年5月在海南省人民医院诊治的轻度子痫前期患者30例(轻度子痫前期组),重度子痫前期患者27例(重度子痫前期组),同期住院分娩的正常晚期妊娠孕妇30例作为对照组。检测3组孕妇外周血CD80、TNF-α、IL-4 mRNA和蛋白的表达水平并分析。结果(1)轻度子痫前期组及重度子痫前期组患者外周血CD80和TNF-α蛋白表达较对照组显著升高(P<0.05),而外周血IL-4蛋白的表达水平较对照组明显降低(P<0.05)。(2)轻度子痫前期组及重度子痫前期组患者外周血单个核细胞CD80和TNF-αmRNA含量较对照组显著升高(P<0.05),而外周血单个核细胞IL-4mRNA含量较对照组明显降低(P<0.05)。(3)CD80与TNF-α、IL-4等因子的表达水平之间有相关性(P<0.05)。CD80与TNF-α水平正相关,与IL-4水平呈负相关。结论CD80表达异常可能与子痫前期的发病有关,其作用机制可能与Th1/Th2的平衡有关。Objective To assess the value of measuring the expression of CD80 and its correlations of TNF-α,and IL-4 in preeclampsia.Methods Altogether,30 patients with mild preeclampsia,27 patients with severe preeclampsia and 30 case of normal pregnancy were recruited.mRNA and protein expression levels of CD80,TNF-αand IL-4 were detected by qRT-PCR and ELISA.Result(1)The protein of CD80 and TNF-αincreased significantly(P<0.05),while the expression level of IL-4 decreased significantly in mild and severe preeclampsia(P<0.05).(2)The mRNA of CD80 and TNF-αincreased significantly in peripheral blood mononuclear cell of mild and severe preeclampsia,while the expression level of IL-4 decreased significantly.(3)There was a correlation among CD80,TNF-αand IL-4(P<0.05).CD80 were positively correlated with TNF-a,but negatively with IL-4 levels.ConclusionCD80 may cause the occurrence of preeclampsia by affecting the balance of Th1/Th2.

关 键 词：CD80 子痫前期 进展 TNF-A IL-4 

分 类 号：R714.244[医药卫生—妇产科学]