去铁胺对大鼠高氧肺损伤的影响  

Effect of deferoxamine on hyperoxic lung injury in rats

作　　者：王晓霞 李玉兰[1] 沙小兰 李二亮 李春兰 Wang Xiaoxia;Li Yulan;Sha Xiaolan;Li Erliang;Li Chunlan(Department of Anesthesiology,First Clinical Medical College of Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州大学第一临床医学院,730000

出　　处：《中华麻醉学杂志》2019年第8期931-934,共4页Chinese Journal of Anesthesiology

摘　　要：目的评价去铁胺对大鼠高氧肺损伤的影响。方法成年雄性SD大鼠24只,体重170~230 g,按随机数字表法分为3组(n=8):高氧组(H组)、去铁胺组(D组)及空气对照组(C组)。气管插管术后行机械通气,C组吸入空气,H组和D组吸入90%氧气。在机械通气同时,D组经尾静脉连续输注去铁胺50 mg·kg^-1·h^-1 4h,共4 ml。H组和C组输注等体积生理盐水。机械通气4 h时,取肺组织,观察病理学结果,行病理学评分;测定肺组织湿重/干重(W/D)比值;采用ELISA法测定肺组织和支气管肺泡灌洗液(BALF)肺表面活性蛋白C(SP-C)、黄嘌呤氧化酶(XOD)和谷胱甘肽还原酶(GR)水平。结果与C组比较,H组和D组肺组织病理学评分和W/D比值升高,肺组织和BALF SP-C和GR水平降低,XOD水平升高(P<0.05);与H组比较,D组肺组织病理学评分和W/D比值降低,肺组织和BALF SP-C和GR水平升高,XOD水平降低(P<0.05)。结论去铁胺可抑制氧化应激反应,减轻大鼠高氧肺损伤。Objective To evaluate the effect of deferoxamine on hyperoxic lung injury in rats.Methods Twenty-four adult male Sprague-Dawley rats,weighing 170-230 g,were divided into 3 groups(n=8 each)using a random number table method:hyperoxia group(group H),deferoxamine group(group D)and air control group(group C).Mechanical ventilation was performed after tracheal intubation,group C inhaled air,and H and D groups inhaled 90%oxygen.Deferoxamine 50 mg·kg^-1·h^-1 was continuously infused via the tail vein for 4 h via the tail vein at the same time of mechanical ventilation in group D.The equal volume of normal saline was infused in H and C groups.At 4 h of mechanical ventilation,lungs were removed for examination of pathological changes of lung tissues and for determination of wet/dry weight ratio(W/D ratio)and levels of pulmonary surfactant protein C(SP-C),xanthine oxidase(XOD)and glutathione reductase(GR)in lung tissues and broncho-alveolar lavage fluid(BALF)were detected by enzyme-linked immunosorbent assay.Results Compared with group C,the pathological score of lung tissues and W/D ratio were significantly increased,the levels of SP-C and GR in lung tissues and BALF were decreased,and the level of XOD in lung tissues and BALF was increased in H and D groups(P<0.05).Compared with group H,the pathological score of lung tissues and W/D ratio were significantly decreased,the levels of SP-C and GR in lung tissues and BALF were increased,and the level of XOD in lung tissues and BALF was decreased in group D(P<0.05).Conclusion Deferoxamine can inhibit oxidative stress response and alleviate hyperoxic lung injury in rats.

关 键 词：去铁胺 高氧症 肺损伤 

分 类 号：R28[医药卫生—中药学]