系统性红斑狼疮患者肠道菌群变化的研究  

Changes of intestinal flora in patients with systemic lupus erythematosus

作　　者：孙志东 魏枫 徐华锋 周海舟[2] Sun Zhidong;Wei Feng;Xu Huafeng;Zhou Haizhou(Medical Department,960 Hospital of the Chinese People's Liberation Army Jinan 250031,China;Department of Laboratory Diagnosis,the First Affifiliated Hospital of Harbin Medical University,Harbin 150001,China;Department of Radio-Immunity,Provincial Hospital,Harbin 150081,China)

机构地区：[1]中国人民解放军第960医院卫勤部,济南250031 [2]哈尔滨医科大学附属第一医院检验科,150001 [3]黑龙江省医院放射免疫特检科,哈尔滨150081

出　　处：《国际免疫学杂志》2019年第6期579-582,共4页International Journal of Immunology

基　　金：黑龙江省卫生计生委科研课题(2017-459)。

摘　　要：目的分析黑龙江省系统性红斑狼疮(systemic lupus erythematosus,SLE)患者肠道菌群的变化,为该病与肠道菌群相关性研究提供一定的数据支持.方法采集12例SLE患者和12名健康人的粪便,并提取粪便标本中的细菌DNA、然后扩增细菌16S RNA,通过测序获取每个细菌的序列,最后进行生物信息分析和SPSS17软件进行统计学分析.结果SLE患者肠道菌群与正常人存在着明显的差异,包括2个菌门(P值分别为0.032,0.029),2个菌科(P值分别为0.029,0.013)和11个菌属(P值分别为0.042,0.049,0.047,0.040,0.028,0.039,0.003,0.046,0.048,0.045,0.049).而且SLE患者肠道菌群的丰度和谱系多样性明显比正常人的肠道菌群减少(P<0.05),而SLE患者肠道菌群的菌群多样性和均匀性与正常人没有差别(P>0.05).结论拟杆菌门和普氏菌属不适合用作SLE疾病诊断和鉴别诊断的菌种;疣微菌科是不受地域饮食和人种影响的一个菌科,它可能在SLE的发生发展中起到重要的作用;11个明显变化菌属可以用来鉴别SLE患者和健康人.Objective To investigate the changes of intestinal flora in patients with systemic lupus erythematosus(SLE)in Heilongjiang province,and to provide some data support for the study of the correlation between SLE and intestinal flora.Methods The feces of 12 patients with SLE and 12 healthy persons were collected.The bacterial DNA was extracted from the fecal samples and the 16s RNA of bacteria was amplified to obtain the sequence of each bacterium by sequencing.Finally,biological information analysis and statistical analysis.SPSS 17 software was used for statistical analysis.Results There were significant differences in intestinal flora between SLE patients and normal subjects,including 2 phylum(P=0.032,0.029),2 families(P=0.029,0.013)and 11 genera(P=0.042,0.049,0.047,0.040,0.028,0.039,0.003,0.046,0.048,0.045 and 0.049).The abundance and phylogenetic diversity of intestinal flora in patients with SLE were significantly lower than those in normal subjects(P<0.05),but the community diversity and evenness of intestinal flora in patients with SLE were not different from those in normal subjects(P>0.05).Conclusions Bacteroidetes and Prevotella may not be suitable for disease diagnosis and differential diagnosis.Ruminococcaceae was a family of microbes which is not affected by regional diet and race.It may play an important role in the development of SLE.Eleven significantly altered genera in SLE patients can be used to distinguish SLE patients from healthy people.

关 键 词：系统性红斑狼疮 肠道菌群 16S RNA 

分 类 号：R59[医药卫生—内科学]