β1-肾上腺素能受体兴奋加重布比卡因心肌细胞线粒体水肿:大鼠实验研究  

Excitation of β1-adrenergic receptor to worsen myocardium mitochondrial edema induced by bupivacaine: a rat experiment

作　　者：张颖颖 段然 程艳欣[3] 赵森明[3] 刘彦辉[1] Zhang Yingying;Duan Ran;Cheng Yanxin;Zhao Senming;Liu Yanhui(Department of Anesthesiology,Third Hospital of Hebei Medical University,Shijiazhuang City,Hebei Province 050051,China;Department of Anesthesiology,Hebei People's Hospital,Shijiazhuang City,Hebei Province 050051,China;Department of Pain Medicine,Third Hospital of Hebei Medical University,Shijiazhuang City,Hebei Province 050051,China)

机构地区：[1]河北医科大学第三医院友谊院区麻醉科,石家庄市050051 [2]河北省人民医院麻醉科,石家庄市050051 [3]河北医科大学第三医院疼痛科,石家庄市050051

出　　处：《实用疼痛学杂志》2019年第5期336-341,共6页Pain Clinic Journal

摘　　要：目的探讨β1-肾上腺素能受体激动剂异丙肾上腺素对布比卡因致心肌细胞线粒体水肿的影响和机制。方法健康清洁级雄性SD大鼠6只,体重180~220 g,采用酶解法分离SD大鼠心肌细胞,将心肌细胞随机分为4组,分别在含生理盐水、布比卡因、布比卡因+异丙肾上腺素、布比卡因+异丙肾上腺素+艾司洛尔的台式液中孵育,并在电刺激下诱导细胞规律收缩60 s后,电镜下观察线粒体形态学改变;使用活性氧检测试剂盒定量检测上述心肌细胞线粒体活性氧(ROS)生成量(均重复3次,取平均值)。结果布比卡因组线粒体水肿程度评分及ROS生成量明显高于生理盐水组(4 719.95比3 921.94)(P均<0.05);布比卡因+异丙肾上腺素组细胞线粒体水肿程度评分及ROS生成量明显高于布比卡因组(5 192.25比4 719.95)(P均<0.05);而布比卡因+异丙肾上腺素+艾司洛尔组线粒体水肿程度评分及ROS生成量明显低于布比卡因+异丙肾上腺素组(4 709.68比5 192.25)(P均<0.05)。结论异丙肾上腺素引起的β1肾上腺素能受体兴奋可干扰线粒体代谢,加重布比卡因导致的心肌细胞线粒体水肿。Objective To investigate the effect and mechanism of isoprenaline,aβ1-adrenergic receptor agonist,on mitochondrial edema of cardiomyocytes induced by bupivacaine.Methods Six healthy pathogen-free male SD rats,weighing 180-220 g,were received enzymatic hydrolysis separation for myocardial cells,which were randomly divided into 4 groups:physiological saline group(control group),bupivacaine group,bupivacaine+isoprenaline group,and bupivacaine+isoprenaline+esmolol group.The myocardial cells were electrically stimulated to induce rhythmic contraction for 60 s.Electron microscopy of myocardial mitochondria was performed to assess the damage of the structure.The amount of mitochondrial reactive oxygen species(ROS)was quantitatively measured by ROS assay kit in the above cardiomyocytes.(Repeat 3 times and take the average).Results The mitochondrial edema score and ROS production was significantly higher in bupivacaine group than those in control group(4719.95 vs.3921.94)(all P<0.05).The extent of myocardial mitochondrial edema and ROS production were significantly higher in bupivacaine+isoprenaline group than those in bupivacaine group(5192.25 vs.4719.95)(all P<0.05).The mitochondrial edema score and ROS production was significantly lower in bupivacaine+isoprenaline+esmolol group than those in bupivacaine+isoprenaline group(4709.68 vs.5192.25)(all P<0.05).Conclusion The excitation ofβ1-adrenergic receptor induced by isoprenaline can interfere with mitochondrial metabolism and aggravate mitochondrial edema of cardiomyocytes caused by bupivacaine.

关 键 词：电刺激 受体 肾上腺素能β1 布比卡因 心肌 线粒体 

分 类 号：R54[医药卫生—心血管疾病]