A Sensitive Fluorescent Turn-on Probe NapP-deap Based on Naphthalimide Derivative to Detect Hg(II) Ions in HEPES Buffer Solution and Living Cells  

作　　者：WANG Shuxin CAO Jian CHENG Yuxiao LU Chenhong 

机构地区：[1]School of Chemistry^and Chemical Engineering,Shanghai University of Engineering Science,Shanghai 201620,P.R.China [2]Technical Center for Industrial Product and Raw Material Inspection and Testing,Shanghai Customs,Shanghai 200135,P.R.China

出　　处：《Chemical Research in Chinese Universities》2019年第6期967-971,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Key Research and Development Program of China(No.2016YFF0203704);the Innovation Fund for Graduate Students of Shanghai University of Engineering Science,China(Nos.l 8KY0416,16KY0410);the National Natural Science Foundation of China(No.21404068).

摘　　要：A highly sensitive fluorescent“turn-on”probe NapP-deap based on naphthalimide derivative was developed that bound Hg^2+ ions rapidly in the N-2-hydroxyethylpiperazine-N-ethane-sulphonic acid(HEPES)buffer solution via photo-induced electron transfer(PET)being inhibited mechanism.The titration experiment displayed that the emission intensity of NapP-deap at 540 nm was almost linearly increased by about 3-fold.The Job’s plot showed a stoichiometry factor of 1:1 of the ligand-to-metal ratio.The detection limit of fluorescent probe was calculated to be 6.2×10^?9 mol/L.^1H NMR studies could confirm that one Hg^2+ ion was bound by the N atoms(a,b)of piperazine or the N atom(c)of pyridine.The fluorescent probe could be used for the detection of Hg^2+ ions in living cells.

关 键 词：FLUORESCENT “turn-on” HG^2+ HEPES buffer solution Living cell PET mechanism 

分 类 号：O62[理学—有机化学]