SXT/R391元件核心基因的生物信息学分析  

Bioinformatics Analysis on the SXT/R391 ICEs Core Genome

作　　者：张琪 周静 周江林 孔娜 胡明达 彭小川 李北平 梁龙 靳远 任洪广 岳俊杰 ZHANG Qi;ZHOU Jing;ZHOU Jiang-Lin;KONG Na;HU Ming-Da;PENG Xiao-Chuan;LI Bei-Ping;LIANG Long;JIN Yuan;REN Hong-Guang;YUE Jun-Jie(Beijing Institute of Biotechnology,Beijing 100071,China)

机构地区：[1]军事科学院军事医学研究院生物工程研究所

出　　处：《生物技术通讯》2019年第5期597-603,共7页Letters in Biotechnology

基　　金：国家自然科学基金（31671363,31801096）;国家科技重大专项（2018ZX10101003-001-008）;北京市自然科学基金（7162146）

摘　　要：目的:SXT/R391元件是革兰阴性菌中研究最充分、已知成员最多的整合性接合元件家族,本研究的主要目的是探讨SXT/R391元件核心基因组的适应性进化规律。方法:收集数据库中及文献报道的SXT/R391家族元件数据,并利用已测序的细菌基因组序列预测该家族元件,分析元件核心基因的进化特性。结果:通过文献搜集和预测,共获得83个SXT/R391元件的基因组序列。在52个核心基因中,计算发现22个基因在进化过程中经历了重组,基因分布于SXT/R391元件的4个必需模块。此外,在7个基因中检测到了正选择信号。结论:分析了SXT/R391元件核心基因的进化机制,为了解该家族的进化和扩散奠定了基础。Objective:To conduct adaptive evolution analysis of core genome of SXT/R391,which is one of the largest,diverse and well-studied family of integrating conjugative elements(ICEs)among Gram-negative bacteria.Methods:We used all available SXT/R391 ICEs genomes to expound the genetic diversity at the genome level and then explore the evolutionary mechanisms contribute to its genetic diversity.Results:83 complete genome se⁃quences of SXT/R391 ICEs from different bacterial species and origins were collected.Among the 52 identified core genes,we found that 22 genes had recombination signals and 7 genes had significant evidence of positive selection.The results demonstrated that recombination contributed most to the diversity of SXT/R391 ICEs.Conclusion:We provide an overview of evolution in SXT/R391 ICEs,which will be helpful for understanding the evolutionary dynamics of the SXT/R391 family.

关 键 词：SXT/R391元件 重组分析 正选择分析 

分 类 号：Q811.4[生物学—生物工程]