孕产妇尿中1-羟基芘水平与孕产妇及新生儿hOGG1和XRCC1基因多态性的研究  

Study on the Level of Maternal Urinary 1-Hydroxypyrene and the Polymorphism of Maternal and Neonatal hOGG1 and XRCC1 Genes

作　　者：阿依谢姆古丽·阿力马斯 尔西丁·买买提[1] 鲁英[1] 赵婷婷 ALIMASI Ayixiemuguli;MAIMAITI Erxiding;LU Ying;ZHAO Tingting

机构地区：[1]新疆医科大学公共卫生学院

出　　处：《环境卫生学杂志》2019年第5期425-429,共5页JOURNAL OF ENVIRONMENTAL HYGIENE

基　　金：新疆维吾尔自治区乌鲁木齐市科技攻关项目(G151310003);新疆维吾尔自治区高校科研计划项目(XJEDU2017M019)

摘　　要：目的探讨乌鲁木齐市孕产妇多环芳烃(polyeyclic aromatic hydrocarbons,PAHs)暴露与孕产妇及新生儿hOGG1和XRCC1基因多态性之间的关系。方法采集乌鲁木齐市某家医院115位孕产妇尿液、静脉血及新生儿脐带血,采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)孕产妇与新生儿的hOGG1和XRCC1的基因位点多态性,采用酶水解-液相色谱-质谱联用仪检测孕产妇尿中PAHs内暴露标志物1-羟基芘(1-OH-P)。结果孕产妇尿中1-OH-P浓度为0.340(0.208~0.555)μg/mmol Cr。孕产妇hOGG1326Ser/Cys基因型不同,体内1-OH-P的浓度也不同(P<0.05)。携带不同的XRCC1399Arg/Gln基因型新生儿,其母亲体内1-OH-P的浓度存在统计学差异(P<0.05)。携带不同XRCC1399Arg/Gln基因型的孕产妇,其体内的1-OH-P的浓度差别无统计学意义(P>0.05)。携带不同的hOGG1326Ser/Cys基因型的新生儿,其母亲的体内1-OH-P的浓度无统计学差异(P>0.05)。结论与国内相关研究相比,乌鲁木齐市孕产妇体内的PAHs处于高暴露水平。携带h OGG1326Ser/Cys基因的孕产妇机体在PAHs高暴露环境中DNA损伤更加敏感。Objectives To investigate the relationship between maternal polycyclic aromatic hydrocarbon(PAHs)exposure in Urumqi and the polymorphisms of maternal and neonatal hOGG1 and XRCC1 genes.MethodsA total of 115 maternal urine,venous blood and neonatal cord blood were collected from a hospital in Urumqi,using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)for gene locus polymorphism of maternal and neonatal hOGG1 and XRCC1.The use of enzymatic hydrolysis-liquid chromatography-mass spectrometry to detect internal exposure marker of PAHs,1-OH-P,in the maternal urine.Results The median level of 1-OH-Pconcentration in maternal urine was 0.340(0.208~0.555)μg/mmol Cr.The maternal hOGG1326 Ser/Cys genotype was different,and the concentration of 1-OH-Pin the body was also different(P<0.05).There was a statistically significant difference in the concentrations of 1-OH-P in the mothers with neonates carrying different XRCC1399 Arg/Gln genotypes(P<0.05).There was no significant difference in the concentration of 1-OH-P between the parturient carrying different XRCC1399 Arg/Gln genotypes(P>0.05).There was no significant difference in the concentration of 1-OH-Pin the mothers with neotates carrying different hOGG1326 Ser/Cys genotypes(P>0.05).Conclusions Compared with domestic related research,PAHs in parturient in Urumqi were at a high the exposure levels.Maternal bodies carrying the hOGG1326 Ser/Cys gene are more susceptible to DNA damage in the high exposure environment of PAHs.

关 键 词：1-羟基芘 X射线交错互补修复基因1 人类8-羟基鸟嘌呤DNA糖苷酶1 

分 类 号：R114[医药卫生—卫生毒理学]