同名经部位对称的少泽、至阴穴刺血法对孕鼠泌乳素、乳腺组织形态及子宫组织Cx-43蛋白表达的影响  

Effects of Bloodletting Needling SI1 and BL67 on Prolactin,Morphology of Breast Tissue and Expression of Cx-43 Protein in Uterine Tissue of Pregnant Rats

作　　者：刘佳 李子腾 郑君[3] LIU Jia;LI Ziteng;ZHENG Jun(Licheng District TCM Hospital of Jinan City,Jinan 250100,China;Xuecheng District TCM Hospital of zaozhuang City,Zaozhuang 277000,China;Shandong University of Traditional Chinese Medicine,Jinan 250013,China)

机构地区：[1]济南市历城区中医医院,山东济南250100 [2]枣庄市薛城区中医院,山东枣庄277000 [3]山东中医药大学,山东济南250013

出　　处：《针灸临床杂志》2019年第12期58-62,共5页Journal of Clinical Acupuncture and Moxibustion

基　　金：山东省自然科学基金，编号:ZR2014HM028

摘　　要：目的:探讨同名经部位对称的少泽、至阴穴刺血法对孕鼠泌乳素、乳腺组织形态的影响及子宫组织Cx-43蛋白的表达,观察部位对称的同名经穴是否存在协同作用。方法:将68只Wistar大鼠(雌鼠50只,雄鼠18只)雌雄合笼制备孕鼠模型,将36只孕鼠随机分为模型组A1、A2、A3,模型对照组B,每组9只;空白对照组C未孕鼠9只,备用5只,模型A1组刺少泽穴、A2组刺至阴穴、A3组刺少泽、至阴穴,模型B组为只孕不针组,空白对照组为不孕不针组,针刺7天,第8天采血后用化学发光法检测各组大鼠血清泌乳素含量,观察乳腺组织形态变化及子宫组织Cx-43蛋白的表达。结果:空白对照组C不孕鼠的泌乳素水平最高,孕鼠模型组A各组及模型对照组B泌乳素水平均有不同程度下降;模型组A各组较模型对照组B泌乳素水平均有不同程度升高,差异具有统计学意义(P<0.05);模型A组各组组间比较泌乳素水平差异无统计学意义(P>0.05);空白对照组C大鼠乳腺小叶腺泡数少、大小不一,腺泡切面相对较小;模型组乳腺小叶腺泡数量增多,腺泡较为规则,腺泡切面相对较大,且模型A组各组较模型对照组B腺泡数量增多,腺泡更为规则;模型组A组组间无明显差异;模型A组各组与模型对照组B的灰度值相比均具有不同程度的升高。结论:刺血法对孕鼠PRL水平有一定影响,呈增高趋势,符合正常孕期的激素水平变化状态,且刺血少泽穴、至阴穴对孕鼠PRL值水平的表达、乳腺组织形态变化及子宫组织Cx-43蛋白的表达有一定的相关性。Objective:To investigate the effect of bloodletting needling points of SI1 and BL67,which are two points located at the symmetrical parts of the meridian bearing the same name,on prolactin,morphology of breast tissue and the expression of Cx-43 protein in uterine tissue of pregnant rats,and to observe whether there is synergy between the points from the meridian bearing the same name.Methods:68 Wistar rats(50 female,18 male)were used to prepare a pregnant rat model.36 pregnant rats were randomly divided into the model groups A(A1,A2,and A3)and the model control group B,with 9 rats in each group;there were 9 pregnant rats in the blank control group,and 5 rats were taken as the spare ones.In the model group A1 SI1 was intervened,BL67 was intervened in group A2,Both SI1 and BL67 were intervened in group A3.The rats in the model control group were not given acupuncture after pregnancy,and the blank control group was the non-pregnant and non-acupuncture group.The contents of serum prolactin were measured by chemiluminescence after acupuncture for 7 days.The morphology changes of mammary tissue and the protein expression of Cx-43 in uterine tissue were observed.Results:The highest levels of prolactin were in group C,in groups A and group B,the levels of prolactin decreased in different degrees in the other groups;compared to group B,prolactin levels in groups A were significantly higher(P<0.05);there were no statistical differences in prolactin levels among the model groups A(P>0.05).The number of acini in the lobule of mammary glands was less,the acini size was varied,and acini section was quite small in group C;the number was increased,the acini size was quite regular,and the acini section was relatively large in the model groups,especially in the groups A;there were no significant difference among groups A.The gray value of the model groups A and the model group B had different degree of increase.Conclusion:Bloodletting acupuncture has a certain effect on PRL level in pregnant rats,with PRL level showing an increasi

关 键 词：同名经 部位对称 少泽穴 至阴穴 刺血 泌乳素 

分 类 号：R246.3[医药卫生—针灸推拿学]