香蕉枯萎病菌4号生理小种smy1基因的功能分析  

Effects of smy1 Gene Knockout on Physiological Function of Fusarium oxysporum f. sp. cubense race 4

作　　者：何壮 漆艳香[2] 曾凡云[2] 丁兆建[2] 梁峻玮 张欣 彭军[2] 谢培兰 谢艺贤[2] HE Zhuang;QI Yanxiang;ZENG Fanyun;DING Zhaojian;LIANG Junwei;ZHANG Xin;PENG Jun;XIE Peilan;XIE Yixian(Institute of Tropical Agriculture and Forestry,Hainan University,Haikou,Hainan 570228,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China)

机构地区：[1]海南大学热带农林学院,海南海口570228 [2]中国热带农业科学院环境与植物保护研究所,海南海口571101

出　　处：《热带作物学报》2019年第12期2447-2455,共9页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.31661143003);中国热带农业科学院托举工程项目(No.1630042018010);现代农业产业技术体系建设专项(No.CARS-31-07)

摘　　要：Smy1是一种参与调控真菌生长的驱动蛋白。本研究克隆了香蕉枯萎病菌4号生理小种(Foc 4)驱动蛋白基因smy1,并采用同源重组技术获得smy1敲除的野生型Foc 4的smy1敲除突变体。通过测定突变体菌丝形态、生长速度、产生孢子量、对香蕉苗的致病力及对氰烯菌酯的敏感性,用于研究smy1在香蕉枯萎病菌的生长发育及对氰烯菌酯抗药性中的作用。结果表明,与野生型Foc 4相比,smy1敲除突变体生长缓慢、菌丝畸形、产孢量增加,对巴西蕉苗的致病力减弱,但对氰烯菌酯的抗性水平变化不大。由此推断smy1基因可能在Foc 4的生长发育、产孢以及致病力等方面具有重要作用。Smy1 is a kinesin involved in the regulation of fungal growth. In the study, the smy1 kinesin gene of Fusarium oxysporum f. sp. cubense race 4(Foc 4) was cloned. The smy1 knockout mutant of Foc 4 was obtained by homologous recombination technique. Besides observing the mycelial morphological characteristic, growth rate and sporulation of the mutants, pathogenicity to banana seedlings and sensitivity to fungicide were studied in order to reveal the role of smy1 played in the growth and development of F. oxysporum and resistance to fungicide. The results showed that, compared with the Foc 4 wild-type strain, the knockout mutants grew slowly, the mycelial morphology changed, the sporulation increased, and the pathogenicity to banana plantlets seedlings decreased, but the resistance level to fungicide changed little. This indicates that the smy1 gene may play an important role in the growth, sporulation and pathogenicity in Foc 4.

关 键 词：香蕉枯萎病菌 基因敲除 致病力 smy1基因 

分 类 号：S436.681[农业科学—农业昆虫与害虫防治]