生物质炭源可溶性有机物消减土霉素生物毒性的效应比较  

Comparison of Dissolved Organic Matter from Biochar on Attenuating Biotoxicity of Oxytetracycline

作　　者：葛成军 赵媛媛[2] 俞花美 岳林[2] 傅悦 马瑞阳 程磊 GE Chengjun;ZHAO Yuanyuan;YU Huamei;YUE Lin;FU Yue;MA Ruiyang;CHENG Lei(College of Life Science,Zhejiang University,Hangzhou,Zhejiang 310058,China;College of Ecology and Environment,Hainan University,Haikou,Hainan 570228,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China)

机构地区：[1]浙江大学生命科学学院,浙江杭州310058 [2]海南大学生态与环境学院,海南海口570228 [3]中国热带农业科学院环境与植物保护研究所,海南海口571101

出　　处：《热带作物学报》2019年第12期2489-2497,共9页Chinese Journal of Tropical Crops

基　　金：海南省重大科技计划项目(No.ZDKJ2017002);国家自然科学基金项目(No.41867045,No.41763015)

摘　　要：为探究生物质炭源溶解性有机物(DOM)对土霉素生物毒性的影响,采用水溶液和土壤模拟培养方式,研究不同温度(350℃、500℃、650℃)制备的木薯渣基生物质炭中溶解性有机物对土霉素胁迫小白菜(Brassica rapa var.glabra)种子发芽、根伸长和芽伸长的影响。结果表明,DOM350和DOM500分别对水培和土培种子的发芽率呈现促进作用,促进率分别为3.33%和10.00%;生物质炭源DOM对土霉素胁迫种子发芽存在缓解作用(P<0.05);土霉素胁迫下DOM浓度与小白菜种子芽伸长呈显著相关(P<0.05);土培条件下,DOM与低浓度土霉素(<34.78 mg/kg)共同促进种子根、芽伸长,对高浓度土霉素(>94.09 mg/kg)存在缓解作用;水培条件下,低浓度DOM对土霉素胁迫种子(B. rapa var. glabra)根伸长呈现缓解作用,DOM浓度与种子芽伸长呈极显著正相关(P<0.01)。In order to explore and state the possibility effects of dissolved organic matter(DOM) on the biotoxicity of oxytetracycline(OTC), aqueous solution and soil culture incubation experiments were conducted to investigate the effects of DOM extracted from biochar, derived from the cassava residue at 350 ℃, 500 ℃, 650 ℃, on the pakchoi(Brassica rapa var. glabra) seed germination, root and bud elongation. Results showed that DOM350 and DOM500 had a promoting effect on seed germination rate of Brassica rapa var. glabra in hydroponic and soil culture condition, which was 3.33% and 10.00%, respectively. For DOM-OTC system, there was a significant correlation between DOM concentrations and bud elongation of pakchoi(B. rapa var. glabra) seeds(P<0.05). Furthermore, DOM had a synergistic effect with low concentration(<34.78 mg/kg) of OTC to promote root and bud extension of seeds, and antagonized the biotoxicity of OTC with high concentration(>94.09 mg/kg) of OTC. However, DOM alleviated the toxicity of OTC on root elongation rate, and had a highly significant effect on shoot elongation of seeds cultured in water(P<0.01), when DOC concentration at 10 mg/L.

关 键 词：溶解性有机物 土霉素 小白菜 生物毒性 生物质炭 

分 类 号：X53[环境科学与工程—环境工程]