Physiological Characteristics of Boscalid Against Botrytis cinerea from Strawberry  

作　　者：Li Pei-qian Feng Bao-zhen Sun Nu-nu 

机构地区：[1]Department of Life Sciences, Yuncheng University

出　　处：《Journal of Northeast Agricultural University(English Edition)》2019年第4期23-30,共8页东北农业大学学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(31501665);the Program for the Scientific and Technologal Innovation Programs Higher Education Institutions in Shanxi(2019L0849,2019L0851);the Opening Foundation of Key Laboratory of Environment Change and Resources Use in Beibu Gulf Ministry of Education(Nanning Normal University);Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation(Nanning Normal University)(NNNU-KLOP-K1904)

摘　　要：A total of 96 monoconidial Botrytis cinerea strains were isolated from strawberry field.Physiological characteristics and resistance analysis to boscalid were investigated.Most isolates showed resistant phenotype and the resistance frequency of highly resistant isolates was as high as 45.10%.The effective concentration that inhibited conidia germination by 50% relative to the control values for sensitive isolates ranged from 0.068 to 60.56μg·mL^-1 in conidia germination assays.Compared to the sensitive strains,the boscalid-resistant strains had a significant increase in phenylalanine ammonia lyase(PAL)and peroxidase(POD)activity and more sensitivity to boscalid pressure.Fitness analysis showed statistically significant difference between sensitive and resistance isolates.Sequencing of SdhB gene indicated that a single mutation corresponded to His272.The results suggested that resistance to boscalid in B.cinerea populations could result in failure to control gray mold.These results would enrich the understanding of the resistance mechanism of B.cinerea to boscalid.A total of 96 monoconidial Botrytis cinerea strains were isolated from strawberry field.Physiological characteristics and resistance analysis to boscalid were investigated.Most isolates showed resistant phenotype and the resistance frequency of highly resistant isolates was as high as 45.10%.The effective concentration that inhibited conidia germination by 50% relative to the control values for sensitive isolates ranged from 0.068 to 60.56 μg · mL-1 in conidia germination assays.Compared to the sensitive strains, the boscalid-resistant strains had a significant increase in phenylalanine ammonia lyase（PAL） and peroxidase（POD） activity and more sensitivity to boscalid pressure.Fitness analysis showed statistically significant difference between sensitive and resistance isolates.Sequencing of Sdh B gene indicated that a single mutation corresponded to His272.The results suggested that resistance to boscalid in B.cinerea populations could result in failure to control gray mold.These results would enrich the understanding of the resistance mechanism of B.cinerea to boscalid.

关 键 词：Botrytis cinerea BOSCALID RESISTANCE 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]