检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:尹跃[1] 赵建华[1] 何昕孺[1] 梁晓婕 安巍[1] 秦小雅 曹有龙[1] YIN Yue;ZHAO Jianhua;HE Xinru;LIANG Xiaojie;AN Wei;QIN Xiaoya;CAO Youlong(National Wolfberry Engineering Research Center,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,China)
出 处:《西北农业学报》2019年第12期2027-2034,共8页Acta Agriculturae Boreali-occidentalis Sinica
基 金:国家自然科学基金地区基金(31760218);宁夏农林科学院科技创新先导资金(NKYJ-17-17);第三批宁夏青年科技人才托举工程(TJGC2018022);全产业链创新示范(QCYL-2018-05)~~
摘 要:以黄果枸杞(Lyciumbararumvar.auranticarpum,用P1表示)为父本,北方枸杞(L.chinensevar.potaninii,用P2表示)为母本,杂交获得F1群体91个单株。从66对SSR引物中筛选出具有双亲互补型杂合位点6对引物,对91个F1单株进行杂种鉴定和遗传变异分析。结果表明:6对引物在91个单株进行扩增检测结果中有83个单株在6个SSR位点上表现为双亲互补型杂合位点,可被认定为真杂种,杂种率为91.2%,另外8个单株出现异常SSR基因型,需要结合细胞学进一步验证。UPGMA聚类分析结果显示:在遗传距离为0.712处,83个F1单株被划分为2大类,第Ⅰ大类包括38个F1单株,占45.8%,第二大类包括45个F1单株,占54.2%。杂交后代遗传变异大,遗传多样性丰富。A total of 91 plants of F1 population were obtained by crossing L.bararum var.auranticarpum as a male parent(expressed in P1) with L.chinense var.potaninii as a female parent(expressed in P2).Six pairs of SSR primers with parental complementary heterozygous loci were screened out from 66 pairs of SSR primers for hybridization identification and genetic variation analysis of those 91 plants.Six pairs of primers were used for amplification experiments.The results indicated that 83 of 91 plants showed parental complementary heterozygous loci on these 6 SSR loci with the heterozygous rate of 91.2%,which could be identified as true hybrids.The other 8 plants showed abnormal SSR genotypes,which needed further verification with cytology.Further UPGMA cluster analysis revealed that the 83 plants of F1 population were divided into two groups at genetic distance of 0.712.The first group included 38 plants(45.8%) and the second group included 45 plants(54.2%).Hybrid progenies have great genetic variation and rich genetic diversity.
关 键 词:枸杞 F1群体 杂种鉴定 SSR标记 遗传多样性
分 类 号:S567.19[农业科学—中草药栽培]
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.200