鱼胶原构建组织工程软骨的可行性研究  

Fish Collagen in Constructing Tissue-Engineered Cartilage

作　　者：赵少华 王一公 徐勇[2] 段亮[2] ZHAO Shaohua;WANG Yigong;XU Yong;DUAN Liang(Microsurgery of Spine,Spinal Cord,Hand and Foot,Xuchang Central Hospital,Xuchang 461000,China;Department of Thoracic Surgery,Shanghai Pulmonary Hospital,Tongji University School of Medicine,Shanghai 200433,China)

机构地区：[1]许昌市中心医院脊柱脊髓及手足显微外科,河南省许昌市461000 [2]上海市肺科医院胸外科,上海市200433

出　　处：《组织工程与重建外科杂志》2019年第6期379-383,共5页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：国家自然科学基金项目（81570089,81970014）;上海市科委项目（19ZR1442600）

摘　　要：目的探索鱼胶原作为支架材料用于构建组织工程软骨的可行性。方法将鱼胶原经冷冻干燥后制备成多孔海绵状支架材料。取兔耳软骨细胞接种于鱼胶原支架,体外培养2周和4周,验证其细胞相容性和体外促进软骨基质分泌的能力。随后植入裸鼠皮下培养6周,行大体观察、组织学检测、生物力学及生物化学定量检测。结果鱼胶原具有良好的细胞相容性,且在体内、外均能形成软骨特异性细胞外基质。体外培养4周和体内培养6周后,HE染色可见典型的软骨陷窝出现。体内培养6周后,Safranin-O染色显示有蛋白聚糖基质产生。生物力学和生物化学结果表明,新生软骨组织均接近正常软骨组织。结论鱼胶原可作为支架材料用于构建组织工程软骨。Objective To explore the feasibility of fish collagen in constructing tissue-engineered cartilage.Methods Porous fish collagen was fabricated by lyophilization.Auricular chondrocytes derived from rabbit were seeded into the porous fish collagen.After cultured in vitro for 2 or 4 weeks,the cytocompatibility and the capacity of cartilage-specific extracellular matrix formation based on fish collagen were investigated.Thereafter,the cell-scaffold constructs were implanted into the rude mice subcutaneously.Gross observation,histological observation and quantitative evaluation of neocartilage test were carried out after cultured for 6 weeks.Results Fish collagen possessed satisfactory cytocompatibility,and facilitated cartilage-specific extracellular matrix formation both in vitro and in vivo.Notably,HE staining showed typical mature lacuna after cultured in vitro for 4 weeks or cultured in vivo for 6 weeks.After cultured in vivo for 6 weeks,Safranin-O staining showed abundant proteoglycan,biomechanical and biochemistry analyses indicated that neocartilage was comparable than native cartilage.Conclusion Fish collagen is a promising natural derived material as scaffold in constructing tissue engineering cartilage.

关 键 词：鱼胶原 组织工程 软骨再生 支架 多孔结构 

分 类 号：Q813.12[生物学—生物工程]