巨噬细胞与单核细胞的细胞骨架调控相关的差异基因表达  

Differential Gene Expression Associated with Regulation of Cytoskeleton of Macrophages and Monocytes

作　　者：宋咏刚 胡文慧 余鹏 石玉玲 赵雪[1,2] 胡祖权 王赟[1,2] 曾柱[1,2,3] SONG Yonggang;HU Wenhui;YU Peng;SHI Yuling;ZHAO Xue;HU Zhuquan;WANG Yun;ZENG Zhu(School of Basic Medical Sciences in Guizhou Medical University,Guiyang 550025,Guizhou,China;The Research Center of the Immune Cell and the Anti-Sports Engineering in Guizhou/Key Laboratory of Biological and Medical Engineering,Guizhou Medical University,Guiyang 550025,Guizhou,China;School of Biology and Engineering in Guizhou Medical University,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州医科大学基础医学院,贵州贵阳550025 [2]贵州医科大学贵州省免疫细胞与抗体工程研究中心生物与医学工程重点实验室,贵州贵阳550025 [3]贵州医科大学生物与工程学院,贵州贵阳550025

出　　处：《贵州医科大学学报》2019年第12期1383-1389,共7页Journal of Guizhou Medical University

基　　金：国家自然科学基金(11762006,31771014,31660258,31860262);贵州省自然科学基金资助项目[黔科合基础(2018)1412],[黔(科合平台人才(2016)5676],[黔科合人才团队(2015)4021],[黔科合平台人才(2017)5718];2011协同创新中心[黔教合协同创新字(2015)04];贵州省细胞与基因工程创新群体[黔教合KY字(2016)031]

摘　　要：目的:分析单核细胞(Mo)向巨噬细胞(Mφ)分化的过程中,细胞骨架的调控相关基因及信号通路的表达变化。方法:CEO数据库获得Mo和Mφ的芯片数据,经RStudio软件分析获得差异表达基因(DEGenes),利用STRING在线工具对DEGenes构建蛋白质相互作用网络(PPI),Cytoscap软件筛选出DEGenes的核心模块;对筛选出的DEGenes的核心模块进行GO和KEGG分析。结果:Mφ相对于Mo的差异表达基因共476个,主要涉及的信号通路包括NOD样受体信号通路和吞噬体信号通路,这些通路中CCL18、CXCL8和C3等相关基因与细胞骨架的调控密切相关。结论:Mo向Mφ分化过程中细胞骨架调控相关基因的表达发生了显著改变。Objective:To analyze the expression of genes and signal pathways related to the regulation of cytoskeleton During the differentiation of monocyte(Mo)into macrophage(Mφ).Methods:The chip data of Mo and Mφwere obtained from CEO database,and the different expression genes(DEGenes)were obtained by RStudio software analysis.The core module of the DEGenes was selected by using the STRING online tool to construct the protein interaction network(PPI)and the Cytscap software.The core modules of DEGenes for GO and KEGG were analyzed.Results:A total of 476 genes were expressed in Mφrelative to Mo.The main signal pathway involved the signal pathway of NOD-like receptor and the signal pathway of the phagocytic body.The related genes such as CCL18,CXCL8 and C3 in these pathways were closely related to the regulation of cytoskeleton.Conclusion:The expression of genes related to the regulation of cytoskeleton in the differentiation of Mo into Mφchanges significantly.

关 键 词：巨噬细胞 单核细胞 细胞骨架 生物信息学 基因芯片 

分 类 号：R392[医药卫生—免疫学]