养阴益气合剂对肺纤维化大鼠TGF-β/Smad信号通路的影响  

Effects of Yangyin Yiqi mixture on TGF-β/Smad signal pathway of bleomycin-induced pulmonary fibrosis rats

作　　者：孟丽红 张晓梅 董环 顾潇枫 于小林 刘彧杉 王晨 辛大永 邱新萍 冯京帅 王洪 MENG Lihong;ZHANG Xiaomei;DONG Huan(Shunyi Hospital of Beijing Hospital of Traditional Chinese Medicine,Beijing 101300,China;不详)

机构地区：[1]北京中医药大学第二临床医学院,100029 [2]北京中医药大学东方医院呼吸热病科 [3]北京中医医院顺义医院呼吸科 [4]首都医科大学附属北京中医医院呼吸科 [5]北京中医医院顺义医院

出　　处：《环球中医药》2019年第12期1810-1815,共6页Global Traditional Chinese Medicine

基　　金：北京市自然科学基金(7172247)

摘　　要：目的研究养阴益气合剂对博来霉素诱导的肺间质纤维化大鼠TGF-β/Smad信号通路的影响。方法将120只SPF级Wistar大鼠随机分为空白组、模型组、泼尼松组及养阴益气合剂低、中、高剂量组,采用气管滴注博来霉素的方法复制肺纤维化大鼠模型,造模后第二天各组予相应药物灌胃,连续给药28天。分别于第14天和28天分批处死大鼠,留取肺组织以实时荧光定量PCR法检测转化生长因子-β1(transforming growth factor-β1,TGF-β1)、Smad3及Smad7表达,留取血清以酶联免疫法检测羟脯氨酸(Hydroxyproline,HYP)表达。结果与空白组相比,模型组TGF-β1、Smad3 mRNA及HYP表达明显升高,Smad7显著减少(P<0.05)。治疗后,肺纤方中、高剂量组TGF-β1、Smad3及HYP出现显著下降,Smad7表达上调(P<0.05)。结论养阴益气合剂的抗肺纤维化作用可能与抑制TGF-β/Smad通路,减少细胞外基质沉积有关。Objective To investigate the effects of Yangyin Yiqi mixture on transforming growth factor-beta/Smad signal pathway of bleomycin-induced pulmonary fibrosis rats.Methods 120 Wistar rats were randomly divided into 6 groups:normal control group,model group,prednisone group,low-dose,mid-dose,and high-dose Yangyin Yiqi mixture groups.Bleomycin was used to establish the pulmonary fibrosis model rats.From the second day after modeling,each group was given a corresponding drug gavage for 28 days.The rats were killed on the 14th and 28th day respectively.The lung tissues were collected to detective the mRNA expression of transforming growth factor-beta1,Smad3 and Smad7 by realtime-PCR method.Serum was collected to detective the expression of hydroxyproline by Elisa method.Results Compared with the normal group,the expression of TGF-β1,Smad3 and HYP in the model group significantly increased,and Smad7 significantly decreased(P<0.05).After treatment,the expression of TGF-β1,Smad3 and HYP in the middle and high dose groups of Yangyin Yiqi mixture decreased significantly,and Smad7 expression was up-regulated(P<0.05).Conclusion The anti-pulmonary fibrosis effect of Yangyin Yiqi mixture may be related to inhibiting TGF-β/Smad pathway and reducing extracellular matrix deposition.

关 键 词：养阴益气合剂 肺纤维化 TGF-β/Smad通路 

分 类 号：R285.5[医药卫生—中药学]