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作 者:杨婷[1] 王亚[1] 张素勤[1] 陈星灼 耿广东[1] 陈丽 YANG Ting;WANG Ya;ZHANG Su-qin;CHEN Xing-zhuo;GENG Guang-dong;CHEN Li(College of Agriculture,Guizhou University,Guiyang 550025,China;Agricultural Park of Puding,Anshun 562100,Guizhou,China)
机构地区:[1]贵州大学农学院,贵阳550025 [2]普定县农业示范园区,贵州安顺562100
出 处:《湖北农业科学》2019年第23期215-217,226,共4页Hubei Agricultural Sciences
基 金:贵州省科技厅重大专项计划项目(黔科合重大专项字[2016]3002号);贵州省农业攻关项目(黔科合支撑[2016]2603号;黔科合支撑[2018]2330号)
摘 要:采用滴片技术获得佛手瓜(Sechium edule Swartz)根尖细胞染色体片子,并进行染色体核型分析,以探索佛手瓜染色体核型特点,为佛手瓜及相近植物遗传育种提供细胞学依据。结果显示,佛手瓜具有14对染色体,均为中部着丝粒染色体(m),每条染色体上均未发现随体,其核型公式为2n=2x=28=28m;核型不对称系数为53.55%,属于对称核型;佛手瓜的核型为1A,是一种进化程度低的葫芦科蔬菜作物。To provide cytological basis for genetics and breeding for Sechium edule Swartz and its similar plants,chromosome preparations from root tip cell of Sechium edule Swartz were obtained by using dropping slide technique,and its karyotype was analyzed in order to understand chromosomal karyotype characteristics.The result showed that the genome of Sechium edule Swartz possessed 14 pairs of chromosomes,all of which were metacentric chromosomes(m),and no any satellite was found in them.Its karyotype formula was 2 n=2 x=28=28 m.The asymmetrical karyotype coefficient was 53.55%,which was symmetrical karyotype.Its karyotype belonged to the type 1 A.Therefore,the evolution degree of Sechium edule Swartz was low among Cucurbitace.
关 键 词:佛手瓜(Sechium edule Swartz) 染色体 核型
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