古籍《中庸大学》污染霉菌的鉴定及其纸质样品霉斑的祛除  

Identification of the Contaminated Fungus in the Ancient Books “ZhongyongDaxue” and the Removal of Mildew from Paper Samples

作　　者：李其久[1] 陈娟 熊丽星 郭旭[2] 耿俊强 张宝珠[3] 张可心[4] 贲松彬[1] LI Qi-jiu;CHEN Juan;XIONG Li-xing;GUO Xu;GENG Jun-qiang;ZHANG Bao-zhu;ZHANG Ke-xin;BEN Song-bin(College of Life Science,Liaoning University,Shenyang 110036,China;Shenyang Product Quality Supervision and Inspection Institute,Shenyang 110022,China;Liaoning University Library,Shenyang 110036,China;Archives Center of Liaoning University,Shenyang 110036,China)

机构地区：[1]辽宁大学生命科学院,辽宁沈阳110036 [2]沈阳产品质量监督检验院,辽宁沈阳110022 [3]辽宁大学图书馆,辽宁沈阳110036 [4]辽宁大学档案中心,辽宁沈阳110036

出　　处：《辽宁大学学报（自然科学版）》2019年第4期294-299,共6页Journal of Liaoning University：Natural Sciences Edition

基　　金：辽宁省科学事业公益研究基金项目(2016003007);辽宁省档案局科技项目计划(L2017B1)

摘　　要：对古籍《中庸大学》菌斑菌株复苏分离鉴定,采用菌落形态特征和显微镜观察以及分子生物学鉴定方法鉴定该菌株,同时该菌株在宣纸上模拟菌斑制备霉斑样品,利用水、复配清洗剂、加入抗坏血酸的复配清洗剂分别对霉斑样品进行清洗.最终菌株鉴定结果为里氏木霉(Trichodermareesei),加入抗坏血酸的复配清洗剂清洗效果最好,木瓜酶浓度为3%,抗坏血酸浓度为0.06%,pH为6.0,清洗温度为30.7℃,可以用其对被里氏木霉污染的古籍进行清洗.The plaque strains of the ancient books“Zhongyongdaxue”were isolated and identified.The morphological characteristics of the colonies,microscopic observation and molecular biological identification methods were used to identify the strains.At the same time,the strains were used to simulate the plaques on rice paper to prepare mold samples,using water,The mixture of cleaning agent and ascorbic acid was used to clean the mildew samples;the final strain was identified as Trichodermareesei,and the cleaning agent with ascorbic acid was the best.The papain concentration was 3%,the ascorbic acid concentration was 0.06%,the pH was 6.0,and the cleaning temperature was 30.7℃.It can be used to clean ancient books contaminated with Trichodermareesei.

关 键 词：古籍 霉菌 鉴定 清洗 

分 类 号：Q939.98[生物学—微生物学]