丝胶蛋白/羟基磷灰石/聚己内酯复合支架材料的制备及表征  

Preparation and Characterization of Sericin/Hydroxyapatite/Polycaprolactone Composite Scaffold Materials

作　　者：方艳 徐水[1] 吴婷芳 朱勇[1] FANG Yan;XU Shui;WU Tingfang;ZHU Yong(College of Biotechnology,Laboratory of Fiber Materials of Southwest University,Chongqing 400715)

机构地区：[1]西南大学生物技术学院材料纤维研究室

出　　处：《材料导报》2019年第S02期533-537,共5页Materials Reports

基　　金：2017年度重庆市蚕桑产业发展补助资金项目(CQ2017CSE010)~~

摘　　要：本文基于丝胶、羟基磷灰石,聚己内酯三者作为生物材料的优良性能,采用二次冷冻干燥法制备了一种新型组织工程材料。首先检测了复合支架材料的理化性能,其次研究了该支架材料的形貌结构,最后利用细胞学实验对该材料进行了细胞毒性评价。结果显示,复合材料成型效果好,孔隙分布均匀,孔隙率均高于50%,丝胶蛋白分子呈β折叠结构,随着复合支架材料中PCL比例不断增加,材料的热分解温度提高,热学性能改善,材料的弹性模量逐渐升高,说明PCL起到了增强支架材料刚性的作用。细胞毒性实验结果表明,细胞生长良好,该复合材料为无毒无害材料。Based on good performance in the application of biomaterial,sericin,hydroxyapatite and polycaprolactone were used to prepare a new type of tissue engineering material via twice lyophilisation.In the experiment,the physical and chemical properties of sericin/hydroxyapatite/polycarprolactone composite scaffold material were studied first;then the morphology of this material was investigated;finally,biocompatibility of this material was evaluated.The results show that the composites have good forming effect,uniform pore distribution and porosity of more than 50%,and the sericin molecules exhibitβ-sheet structure.With the increasing proportion of PCL in the composite scaffold material,the thermal decomposition temperature of the material increase,its performance improved,the elastic modulus of the material gradually increased,indicating PCL has played a role in enhancing the rigidity of the stent material.Cytotoxicity test results show that cells grow well,the composite material is non-toxic and harmless.

关 键 词：丝胶蛋白 羟基磷灰石 聚己内酯 复合支架材料 

分 类 号：TQ341.5[化学工程—化纤工业]