基于羟基氧化铁/石墨烯复合材料构建生物传感器用于ATP的检测  

Biosensor based on FeOOH-rGO composites for the detection of ATP

作　　者：周倩 顾勇军 於霞 卢明华[1] ZHOU Qian;GU Yongjun;YU Xia;LU Minghua(College of Chemistry and Chemical Engineering,Henan University,Kaifeng 475004,Henan,China;Taizhou Urban Construction and Investment Development Group Co.Ltd.,Taizhou 318000,Zhejiang,China;Zhejiang Fuxing Environment Development Co.Ltd.,Taizhou 318000,Zhejiang,China)

机构地区：[1]河南大学化学化工学院,河南开封475004 [2]台州市城市建设投资发展集团有限公司,浙江台州318000 [3]浙江福星环境发展有限公司,浙江台州318000

出　　处：《化学研究》2019年第5期472-477,共6页Chemical Research

基　　金：国家自然科学基金(21477033)

摘　　要：采用FeOOH-rGO复合材料作为光敏材料,并将负载了大量葡萄糖分子的功能化介孔二氧化硅纳米颗粒(MSNs)作为识别探针和信号放大系统构建生物传感平台用于ATP的检测.在目标物存在的情况下,ATP与适配体进行特异性结合,使得MSNs的孔道被打开,葡萄糖分子被释放并作为电子供体参与光电转换过程,引起光电流的增加.随着ATP浓度的增大,释放的葡萄糖分子增加,光电流信号增强,通过监测光电流信号从而实现对目标物ATP浓度的检测.This paper focused on the construction of biosensing platform for ATP detection using FeOOH-rGO composites as photoactive material and glucose-loading MSNs as the recognition probe and signal amplification system.In the presence of ATP,aptamer competitively bind with the target and glucose molecules encapsulated in MSNs could be released.The released glucose molecules served as electron donor to enhance the photocurrent of the system.With the increasing concentration of ATP,more glucose molecules could be released,and higher photocurrents could be obtained.Thus,by monitoring the photocurrents varying with the target,sensitive detection of ATP could be achieved.

关 键 词：光电化学 生物传感 适配体 ATP 

分 类 号：O65[理学—分析化学]