两种绝经后压力性尿失禁大鼠模型的比较研究  

Comparative study on two rat models of postmenopausal stress urinary incontinence

作　　者：单佳汪 银锋 刘晓霞 周勇[2] 张祖娟[2] 李文娟[2] 谢臻蔚[2] Shan Jia;Wang Yinfeng;Liu Xiaoxia;Zhou Yong;Zhang Zujuan;Li Wenjuan;Xie Zhenwei(Department of Gynecology,Shaoxing Maternal and Children′s Hospital,Zhejiang 312000,China)

机构地区：[1]浙江省绍兴市妇幼保健院妇科,312000 [2]浙江大学医学院附属妇产科医院妇科

出　　处：《中国药物与临床》2019年第23期4054-4056,共3页Chinese Remedies & Clinics

基　　金：浙江省医药卫生科技项目(2017203850);浙江省中医药科技计划重点研究项目(2018ZZ017)。

摘　　要：目的比较2种常用绝经后压力性尿失禁动物模型的稳定性。方法48只雌性SD大鼠,随机取12只作为空白组(不做任何处理),剩余36只雌性SD大鼠随机分成3组,A组(对照组):双侧卵巢切除组;B组(阴道气囊扩张组):阴道气囊扩张+双侧卵巢切除;C组(双侧阴部神经离断组):双侧阴部神经离断+双侧卵巢切除。分别在第8周时测定空白组和对照组的卵泡刺激素(FSH)、黄体生成素(LH)和雌二醇(E2)水平,并在建模后第1周、第4周、第8周分别检测膀胱漏点压及喷嚏试验。结果第8周时对照组的FSH和LH值均高于空白组,E2值低于空白组。阴部神经离断组和阴道气囊扩张组在第1周和第4周时的膀胱漏点压均低于对照组,但在第8周时阴道气囊扩张组与对照组的差异无统计学意义,而阴部神经离断组与对照组的差异有统计学意义。喷嚏试验结果显示,建模后第1周,阴部神经离断组和阴道气囊扩张组喷嚏试验阳性率与对照组的差异均有统计学意义;第4周时,仅阴部神经离断组有统计学意义;第8周时,2组与对照组比较差异均无统计学意义。结论双侧阴部神经离断法和阴道气囊扩张法都是绝经后压力性尿失禁动物模型的可行方案,均适用于短期实验研究;而对于长期实验研究,阴部神经离断法较阴道气囊扩张法更稳定。Objective To compare the stability of two commonly used animal models of postmenopausal stress urinary incontinence.Methods Of 48 female Sprague-Dawley rats,12 were randomly selected as the blank group(no treatment),and the remaining 36 were randomly divided into 3 groups:the control group(group A)to receive bilateral ovariectomy,the vaginal balloon dilatation group(group B)to undergo vaginal balloon dilatation+bilateral ovariectomy,and the bilateral pudendal denervation group(group C)to receive bilateral pudendal denervation+bilateral ovariectomy.After 8 weeks,the levels of FSH,LH and E2 in the blank group and the control group were measured.At weeks 1,4 and 8 after modeling,measurement of bladder leak point pressure(BLPP)and sneezing test were performed.Results After 8 weeks,the control group had higher FSH and LH levels and lower E2 level compared with the blank group.The groups B and C showed lower BLPP than those in the control group at weeks 1 and 4.At 8 weeks,the BLPP was comparable between group B and the control group;however,the difference in BLPP between group C and the control group remained statistically significant.In sneezing test,there was a significant difference in the positive rate when comparing the control group with group B or C at 1 week after modeling.After 4 weeks,the difference in the positive rate remained significiantly when comparing the control group with group C.After 8 weeks,there was no difference in the positive rate of sneezing test between any two groups.Conclusion Either bilateral pudendal denervation or vaginal balloon dilatation is feasible for animal modeling of postmenopausal stress urinary incontinence,and is suitable for short-term experiments.For long-term experimental studies,vaginal denervation can be more stable than vaginal balloon dilation for the modeling.

关 键 词：尿失禁 压力性 绝经后期 大鼠 动物 模型 

分 类 号：R71[医药卫生—妇产科学]